Effect of pioglitazone on the fructose-induced abdominal adipose tissue dysfunction.
Effect of pioglitazone on the fructose-induced abdominal adipose tissue dysfunction.
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DOI：
10.1155/2012/259093
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发表时间：
2012
期刊：
影响因子：
2.9
通讯作者：
Spinedi E
中科院分区：
文献类型：
--
作者：
Alzamendi A;Giovambattista A;García ME;Rebolledo OR;Gagliardino JJ;Spinedi E
瞄准。目的：探讨PPARγ在富含果糖饲料喂养正常大鼠3周所致的内分泌腹部组织功能障碍中的作用。方法论。成年正常雄性大鼠接受不含或不含吡格列酮(PIO)的标准商业饮食(CD)或FRD(在饮用水中占10%)(I.P. mg/kg体重/天；CD-PIO和FRD-PIO)。此后，我们测量了循环代谢、内分泌和氧化应激(OS)标志物，腹部脂肪组织(AAT)质量，瘦素(LEP)和纤溶酶原激活物抑制物(PAI-1)组织含量/表达，以及不同浓度胰岛素孵育的分离脂肪细胞的瘦素释放。结果。FRD大鼠的血糖、胰岛素、甘油三酯、TBARS、LEP和PAI-1水平较高；PIO联合给药完全阻止了所有这些增加。FRD大鼠的AAT脂肪细胞更大，分泌更多的LEP，对胰岛素刺激的敏感性降低；PIO显著改善了这些效应。FRD大鼠AAT、LEP和PAI-1(MRNA)浓度显著升高，而胰岛素受体底物(IRS-)1和IRS-2浓度显著降低。PIO联合给药可阻止FRD对AAT中LEP、PAI-1、IRS-2(完全)和IRS-1(部分)mRNAs的影响。结论。PPARγ在FRD所致代谢-内分泌功能障碍的发生发展中可能起到一定的作用。
Aim. To test the potential role of PPARγ in the endocrine abdominal tissue dysfunction induced by feeding normal rats with a fructose rich diet (FRD) during three weeks. Methodology. Adult normal male rats received a standard commercial diet (CD) or FRD, (10% in drinking water) without or with pioglitazone (PIO) (i.p. 0.25 mg/Kg BW/day; CD-PIO and FRD-PIO). Thereafter, we measured circulating metabolic, endocrine, and oxidative stress (OS) markers, abdominal adipose tissue (AAT) mass, leptin (LEP) and plasminogen activator inhibitor-1 (PAI-1) tissue content/expression, and leptin release by isolated adipocytes incubated with different concentrations of insulin. Results. Plasma glucose, insulin, triglyceride, TBARS, LEP, and PAI-1 levels were higher in FRD rats; PIO coadministration fully prevented all these increments. AAT adipocytes from FRD rats were larger, secreted a higher amount of LEP, and displayed decreased sensitivity to insulin stimulation; these effects were significantly ameliorated by PIO. Whereas AAT LEP and PAI-1 (mRNA) concentrations increased significantly in FRD rats, those of insulin-receptor-substrate- (IRS-) 1 and IRS-2 were reduced. PIO coadministration prevented FRD effects on LEP, PAI-1, and IRS-2 (fully) and IRS-1 (partially) mRNAs in AAT. Conclusion. PPARγ would play a relevant role in the development of the FRD-induced metabolic-endocrine dysfunction.
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影响因子：
4.2
作者：
Faure, P;Rossini, E;Halimi, S
通讯作者：
Halimi, S
影响因子：
8.2
作者：
Hajduch, E;Darakhshan, F;Hundal, HS
通讯作者：
Hundal, HS
影响因子：
64.8
作者：
Houstis, N;Rosen, ED;Lander, ES
通讯作者：
Lander, ES
影响因子：
4.5
作者：
Basciano, Heather;Federico, Lisa;Adeli, Khosrow
通讯作者：
Adeli, Khosrow
影响因子：
56.9
作者：
Cohen, B;Novick, D;Rubinstein, M
通讯作者：
Rubinstein, M