Prevotella Induces the Production of Th17 Cells in the Colon of Mice.
Prevotella Induces the Production of Th17 Cells in the Colon of Mice.
复制标题
普雷沃氏菌诱导小鼠结肠中 Th17 细胞的产生。
DOI：
10.1155/2020/9607328
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发表时间：
2020
影响因子：
4.1
通讯作者：
Ma Y
中科院分区：
文献类型：
--
作者：
Huang Y;Tang J;Cai Z;Zhou K;Chang L;Bai Y;Ma Y
Th 17介导的粘膜炎症与普雷沃菌属细菌丰度增加有关。然而，普雷沃氏菌在稳定状态下实际参与肠道Th 17细胞的发育和积累仍然不确定。在此，我们研究了普雷沃氏菌在诱导小鼠肠道Th 17细胞中的作用。小鼠在其饮用水中用广谱抗生素（包括氨苄青霉素、硫酸新霉素、盐酸万古霉素和甲硝唑）的组合处理4周，然后用普雷沃氏菌管饲4周。接种后，16 S rDNA测序用于验证普雷沃氏菌在小鼠结肠中的定殖。通过结肠切片的免疫荧光测定（IFA）定位和定量IL-17 A以及表达IL-17 A的T细胞。采用流式细胞仪对小鼠肠系膜淋巴结中的Th 17细胞进行计数。基于IL-6、TNF-α、IL-1β、IL-17 A、IL-10、IL-4、IFN-γ和IL-2的血清水平，评价了对普雷沃氏菌定殖的全身免疫应答。通过刺激骨髓来源的树突状细胞（BMDCs）来评估由普氏菌诱导的Th 17极化细胞因子（IL-6、TNF-α、IL-1β和IL-2）。结果显示，接种后，普雷沃氏菌成功地定殖在小鼠肠道，并诱导结肠Th 17细胞在结肠中的产生和积累。此外，普雷沃菌还升高了小鼠血清中一些Th 17相关细胞因子的水平。而BMDCs产生的Th 17极化细胞因子（IL-6和IL-1β）主要是通过Prevotella菌与Toll样受体2（TLR 2）的相互作用介导的。总之，我们的数据表明，普雷沃菌诱导小鼠结肠中Th 17细胞的产生，从而突出了普雷沃菌在训练肠道免疫系统中的潜在作用。
Th17-mediated mucosal inflammation is related to increased Prevotella bacterial abundance. The actual involvement of Prevotella in the development and accumulation of intestinal Th17 cells at a steady state, however, remains undefined. Herein, we investigated the role of Prevotella in inducing intestinal Th17 cells in mice. Mice were treated with a combination of broad-spectrum antibiotics (including ampicillin, neomycin sulfate, vancomycin hydrochloride, and metronidazole) in their drinking water for 4 weeks and then gavaged with Prevotella for 4 weeks. After inoculation, 16S rDNA sequencing was used to verify the colonization of Prevotella in the colon of mice. The IL-17A as well as IL-17A-expressing T cells was localized and quantified by an immunofluorescence assay (IFA) of colon sections. Th17 cells in the mesenteric lymph nodes of mice were counted by flow cytometry. Systemic immune response to Prevotella colonization was evaluated based on the serum levels of IL-6, TNF-α, IL-1β, IL-17A, IL-10, IL-4, IFN-γ, and IL-2. Th17-polarizing cytokines (IL-6, TNF-α, IL-1β, and IL-2) induced by Prevotella were evaluated by stimulation of bone marrow-derived dendritic cells (BMDCs). Results revealed that after inoculation, Prevotella successfully colonized the intestine of mice and induced the production and accumulation of colonic Th17 cells in the colon. Moreover, Prevotella elevated some of the Th17-related cytokines in the serum of mice. And Th17-polarizing cytokines (IL-6 and IL-1β) produced by BMDCs were mediated mainly through the interaction between Prevotella and Toll-like receptor 2 (TLR2). In conclusion, our data suggest that Prevotella induces the production of Th17 cells in the colon of mice, thus highlighting the potential role of Prevotella in training the intestinal immune system.
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DOI：
10.1084/jem.20070663
发表时间：
2007-08-06
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Annunziato F;Cosmi L;Santarlasci V;Maggi L;Liotta F;Mazzinghi B;Parente E;Filì L;Ferri S;Frosali F;Giudici F;Romagnani P;Parronchi P;Tonelli F;Maggi E;Romagnani S
通讯作者：
Romagnani S
影响因子：
64.8
作者：
Bettelli, E;Carrier, YJ;Kuchroo, VK
通讯作者：
Kuchroo, VK
影响因子：
17.1
作者：
Faith JJ;Ahern PP;Ridaura VK;Cheng J;Gordon JI
通讯作者：
Gordon JI
DOI：
10.1126/science.1198469
发表时间：
2011-01-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Tanoue T;Shima T;Imaoka A;Kuwahara T;Momose Y;Cheng G;Yamasaki S;Saito T;Ohba Y;Taniguchi T;Takeda K;Hori S;Ivanov II;Umesaki Y;Itoh K;Honda K
通讯作者：
Honda K
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer