GT Transcription Factors of Rosa rugosa Thunb. Involved in Salt Stress Response.
GT Transcription Factors of Rosa rugosa Thunb. Involved in Salt Stress Response.
复制标题
DOI：
10.3390/biology12020176
复制
发表时间：
2023-01-22
期刊：
影响因子：
4.2
通讯作者：
Feng, Liguo
中科院分区：
文献类型：
--
作者：
Wang, Jianwen;Cheng, Yufei;Shi, Xinwei;Feng, Liguo
盐胁迫对植物细胞产生离子毒性，限制了栽培玫瑰的培养。盐胁迫应答中的GT基因不断涌现。从耐盐野生玫瑰GT基因家族中，鉴定出4个盐胁迫响应基因（RrGT-1，RrSIP 1，RrSIP 2，RrGTγ-4）。RrSIP 1和RrGTγ-4、RrGT-1和RrSIP 2分别定位于叶绿体和细胞核。RrSIP 1、RrSIP 2和RrGTγ-4可能参与钠离子和钾离子转运的调控。RrGT-1在野生玫瑰中的特异性表达高于盐敏感栽培玫瑰。这4个基因可作为进一步研究野生玫瑰耐盐性调控机制的候选基因，也可为栽培玫瑰耐盐性改良提供基因资源。玫瑰是一种著名的芳香植物，但由于商品品种耐盐性差，严重影响了其在盐碱地的栽培。在许多植物中，GT（或三螺旋）基因在盐胁迫反应中的作用已逐渐显现。在野外的R. rugosa的37个GT（RrGT）分为GT-1、GT-2、GTγ、SH 4和SIP 1谱系。SIP 1谱系通过转座扩增。参与GT顺式元件结合的基序是保守的。4个RrGT（RrGT 11/14/16/18）在盐胁迫下在根或叶中差异表达显著。NaCl处理8 h内的响应模式表明，RrGTγ-4（RrGT 18）和RrGT-1（RrGT 16）受盐诱导显著。皱纹。RrSIP 1（RrGT 11）和RrGTγ-4定位于叶绿体，RrGT-1和RrSIP 2（RrGT 14）定位于细胞核。RrSIPs和RrGTγ-4对离子转运的调节可能是最重要的作用。RrGT-1可能是一个转录丰度高于糖生GT-1的盐生基因。这些结果为进一步研究RrGTs在盐胁迫响应中的作用提供了重要线索，也将有助于深入理解R.皱纹。
Salt stress produced ion toxicity on plant cells and limited the of culture of cultivated Rosa rugosa. GT genes in salt stresses responses have been emerging. From the GT gene family of the salt-tolerant wild Rosa rugosa, four NaCl stress responsive genes (RrGT-1, RrSIP1, RrSIP2, RrGTγ-4) were identified. RrSIP1 and RrGTγ-4, RrGT-1 and RrSIP2 located on chloroplasts and cell nucleus, respectively. RrSIP1, RrSIP2 and RrGTγ-4 could play roles in regulation of sodion and potassium transport. And RrGT-1 expressed higher specifically in wild Rosa rugosa than in the salt-sensitive cultivated Rosa rugosa. These four genes would be candidates for further study of regulation mechanism of salt-tolerance of wild Rosa rugosa and would supply gene resources for tolerance improvement of cultivated Rosa rugosa. Rosa rugosa was a famous aromatic plant while poor salt tolerance of commercial cultivars has hindered its culture in saline-alkali soil. In many plants, the roles of GT (or trihelix) genes in salt stresses responses have been emerging. In the wild R. rugosa, a total of 37 GTs (RrGTs) were grouped into GT-1, GT-2, GTγ, SH4, and SIP1 lineages. SIP1 lineage expanded by transposition. The motifs involved in the binding of GT cis-elements were conserved. Four RrGTs (RrGT11/14/16/18) significantly differentially expressed in roots or leaves under salt stress. The responsive patterns within 8 h NaCl treatment indicated that RrGTγ-4 (RrGT18) and RrGT-1 (RrGT16) were significantly induced by salt in roots of R. rugosa. Subcellular localizations of RrSIP1 (RrGT11) and RrGTγ-4 were on chloroplasts while RrGT-1 and RrSIP2 (RrGT14) located on cell nucleus. Regulation of ion transport could be the most important role of RrSIPs and RrGTγ-4. And RrGT-1 could be a halophytic gene with higher transcription abundance than glycophytic GT-1. These results provide key clue for further investigations of roles of RrGTs in salt stress response and would be helpful in the understanding the salt tolerance regulation mechanism of R. rugosa.
登录
查看更多内容
影响因子：
5.6
作者：
Mishra A;Tanna B
通讯作者：
Tanna B
影响因子：
2.1
作者：
Osorio MB;Bücker-Neto L;Castilhos G;Turchetto-Zolet AC;Wiebke-Strohm B;Bodanese-Zanettini MH;Margis-Pinheiro M
通讯作者：
Margis-Pinheiro M
影响因子：
7.4
作者：
Park, HC;Kim, ML;Cho, MJ
通讯作者：
Cho, MJ
影响因子：
4.6
作者：
Brewer, PB;Howles, PA;Smyth, DR
通讯作者：
Smyth, DR
影响因子：
2.8
作者：
Kitakura, Saeko;Terakura, Shinji;Machida, Yasunori
通讯作者：
Machida, Yasunori