Quantitative iTRAQ-based proteomic analysis of rice grains to assess high night temperature stress.
Quantitative iTRAQ-based proteomic analysis of rice grains to assess high night temperature stress.
复制标题
基于 iTRAQ 的稻谷定量蛋白质组分析，以评估夜间高温胁迫。
DOI：
10.1002/pmic.201600365
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发表时间：
2017-03
期刊：
影响因子：
3.4
通讯作者：
Huang YJ
中科院分区：
文献类型：
--
作者：
Zhang HY;Lei G;Zhou HW;He C;Liao JL;Huang YJ
水稻产量和品质受到全球地表温度升高的不利影响，并且很大程度上归因于夜间高温（HNT）而不是白天高温。然而，水稻耐热特性的分子机制仍不清楚。在本研究中，我们使用 iTRAQ 方法比较了早期乳熟期水稻耐热和敏感品系的蛋白质组。我们已经鉴定了两个品系之间的 38 个差异表达蛋白，其中 32 个蛋白已在 NCBI 和/或 UniProt 数据库中进行了功能注释。然后将这些蛋白质分为七个功能亚组，包括信号转导、转录调节、氧化、防御反应、运输、能量代谢和生物合成。进一步分析表明，HNT胁迫可以破坏植物细胞的氧化还原平衡，进而触发钙依赖性蛋白激酶和COP9信号体，从而调节参与HNT反应的下游基因/蛋白质。候选蛋白可能为改善水稻耐热特性提供遗传资源，所提出的信号转导和转录调控模型可能有助于阐明水稻响应 HNT 胁迫的分子机制。
Rice yield and quality are adversely affected by increasing global surface temperature, and are strongly attributed to high night temperature (HNT) than high daytime temperature. However, the molecular mechanism underlying the heat‐tolerant characteristics of rice remains unclear. In the present study, we compared the proteomes of heat‐tolerant and ‐sensitive lines of rice at early milky stage using an iTRAQ method. We have identified 38 differentially expressed proteins between the two lines, of which 32 proteins have been functionally annotated in NCBI and/or the UniProt database. These proteins were then classified into seven functional subgroups, which include signal transduction, transcript regulation, oxidation, defense response, transport, energy metabolism, and biosynthesis. Further analysis indicated that HNT stress could disrupt the redox equilibrium of plant cells, which in turn triggers the calcium‐dependent protein kinase and COP9 signalosome, thereby regulating downstream genes/proteins that are involved in the HNT response. The candidate proteins may provide genetic resources for the improvement of heat‐tolerant characteristics in rice, and the proposed model for signal transduction and transcriptional regulation may facilitate in the elucidation of the molecular mechanism underlying the response to HNT stress in rice.
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