Cell-bound and extracellular matrix-associated alkaline phosphatase activity in rat periodontal ligament. Experimental Oral Biology Group.
Cell-bound and extracellular matrix-associated alkaline phosphatase activity in rat periodontal ligament. Experimental Oral Biology Group.
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大鼠牙周膜中细胞结合和细胞外基质相关的碱性磷酸酶活性。
DOI：
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发表时间：
1996
影响因子：
3.5
通讯作者：
W. Beertsen
中科院分区：
文献类型：
--
作者：
M. Groeneveld;T. V. D. Bos;V. Everts;W. Beertsen
以往的研究表明，牙周膜中碱性磷酸酶(ALP)的活性似乎不仅与细胞有关，还可能与细胞外基质有关。为了阐明这一点，我们在电子显微镜水平上研究了该酶的分布。此外，在用(I)溶解膜或裂解磷脂酰肌醇锚(分别为Triton X-100或磷脂酰肌醇-磷脂酶C)和(Ii)基质降解酶鸡尾酒(胶原酶、透明质酸酶和弹性酶)的试剂提取韧带后，对ALP活性进行了生化评估。组织化学观察显示：(A)ALP活性分布不均，在牙槽骨附近活性最高；(B)有两个活性池；一个与细胞结合，另一个与胶原细胞外基质结合。与此相一致的是生化数据表明，大约10%的酶活性牢固地结合在细胞外基质上，90%的酶活性稳定地结合在质膜上。等电聚焦没有显示这两个组分之间的差异，两个样品都产生了一个单一的宽带，对应的等电点约为4.4。
In previous studies it was noted that alkaline phosphatase (ALP) activity in periodontal ligament does not only seem to be related to cells but may also be associated with the extracellular matrix. In an attempt to clarify this we studied the distribution of the enzyme at the electron microscopic level. In addition, ALP-activity was assessed biochemically following extraction of the ligament with (i) agents dissolving the membrane or splitting the phosphatidylinositol anchor (Triton X-100 or phosphatidylinositol-phospholipase C, respectively), and (ii) a matrix-degrading enzyme cocktail (collagenase, hyaluronidase and elastase). Histochemical observations revealed (a) a heterogeneous distribution of ALP-activity, with highest activity adjacent to the alveolar bone and (b) two pools of activity; one bound to cells and one associated with the collagenous extracellular matrix. In line with this were the biochemical data indicating that approximately 10% of the enzyme activity was firmly bound to the extracellular matrix and 90% to plasma membranes. Isoelectric focusing did not reveal differences between the two fractions, both samples yielding a single broad band corresponding with an isoelectric point of about 4.4.
影响因子：
4.3
作者：
ARCEO, N;SAUK, JJ;SOMERMAN, MJ
通讯作者：
SOMERMAN, MJ
甲状旁腺激素刺激成骨细胞样细胞摄取 45Ca2：可能涉及碱性磷酸酶。
影响因子：
4.8
作者：
Fukayama,S;TashjianJr,AH
通讯作者：
TashjianJr,AH
DOI：
--
发表时间：
1988-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Genge;G. R. Sauer;L. N. Wu;F. M. McLean;R. Wuthier
通讯作者：
B. Genge;G. R. Sauer;L. N. Wu;F. M. McLean;R. Wuthier