Conjugated dienes in ischemic and reperfused myocardium: an in vivo chemical signature of oxygen free radical mediated injury.
Conjugated dienes in ischemic and reperfused myocardium: an in vivo chemical signature of oxygen free radical mediated injury.
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缺血和再灌注心肌中的共轭二烯：氧自由基介导的损伤的体内化学特征。
DOI：
10.1016/s0022-2828(87)80596-5
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发表时间：
1987
影响因子：
5
通讯作者：
D. Mickle
中科院分区：
文献类型：
--
作者：
A. Romaschin;I. Rebeyka;G. Wilson;D. Mickle
自由基反应被认为是缺血和再灌注期间心肌损伤机制中的重要事件。大多数涉及心肌损伤病因中自由基介导的事件的体内证据都是基于干预研究，这些研究记录了氧自由基清除剂在改善功能或组织挽救方面的功效。我们测定了源自心肌磷脂的游离脂肪酸氧化产物（羟基共轭二烯）作为氧化损伤的化学标志物。使用 HPLC 分析羟基共轭二烯，并通过 GC-MS 进行结构确认，从没有主动脉交叉钳夹的心肺转流犬心肌（对照）、缺血前、缺血终末（在主动脉交叉钳诱导的全身常温缺血 45 分钟结束时）和再灌注 5、10、15、30 和 60 分钟时采集的活检组织进行羟基共轭二烯分析。所有活检均取自非坏死心肌，如心肌横截面的粗四唑染色所示。在从经受心肺转流术而没有缺血的心脏采集的缺血前活组织检查或对照活组织检查中可以检测到痕量水平的羟基共轭二烯。然而，在 45 分钟缺血结束时，GC-MS 检测到并证实了 18:2 和 20:4 羟基异构体的显着增加（与对照相比，P<0.05）。再灌注 5 分钟后，羟基共轭二烯进一步显着增加，并鉴定出 18:2、20:4 和 22:6 异构体（与缺血结束时相比，P<0.01）。再灌注 30 分钟时，磷脂氧化产物的浓度已恢复至缺血前水平。这项研究首次提供了心肌缺血和再灌注过程中磷脂氧化产物（羟基共轭二烯）形成的严格化学体内证据，并支持氧自由基介导的脂质过氧化的概念。这项研究强调了缺血期间，特别是再灌注早期阶段磷脂氧化产物的形成，并说明了这些产物在心肌磷脂中的瞬时性质。
Free radical reactions have been suggested to be important events in the mechanism of myocardium injury during ischemia and reperfusion. Most of thein vivoevidence implicating free radical mediated events in the etiology of myocardial injury has been based on intervention studies which document the efficacy of oxygen free radical scavengers in improving function or tissue salvage. We have assayed free fatty acid oxidation products (hydroxy conjugated dienes) derived from myocardial phospholipid as chemical markers of oxidative injury. Biopsies, harvested from canine myocardium subjected to cardiopulmonary bypass with no aortic crossclamp (control), from pre-ischemic, end ischemic (at the end of 45 min of global normothermic ischemia induced by aortic crossclamp) and at 5, 10, 15, 30 and 60 mins of reperfusion were analyzed for hydroxy conjugated dienes using HPLC with structural confirmation by GC-MS. All biopsies were taken from non-necrotic myocardium as indicated by gross tetrazolium staining of myocardial cross sections. Trace levels of hydroxy conjugated dienes could be detected in the preischemic biopsies or control biopsies harvested from hearts subjected to cardiopulmonary bypass with no ischemia. At the end of 45 min ischemia, however, a significant increase in 18:2 and 20:4 hydroxy isomers was detected and confirmed by GC-MS (P<0.05 vs. control). After 5 mins of reperfusion a further significant increase in hydroxy conjugated dienes was noted with 18:2,20:4 and 22:6 isomers being identified (P<0.01 vs. end ischemia). By 30 min of reperfusion the concentration of phospholipid oxidation products had returned to pre-ischemic levels. This study presents the first chemically rigorousin vivoevidence for the formation of products of phospholipid oxidation (hydroxy conjugated dienes) during myocardial ischemia and reperfusion and supports the concept of oxygen free radical mediated lipid peroxidation. This study emphasizes the formation of phospholipid oxidation products during ischemia and particularly during the early phase of reperfusion and illustrates the transient nature of these products in myocardial phospholipid.
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由于自由基，增强了溶酶体磷脂的降解和溶酶体磷脂的产生。
DOI：
10.1016/0006-291x(84)90941-0
发表时间：
1984
影响因子：
3.1
作者：
Weglicki,WB;Dickens,BF;Mak,IT
通讯作者：
Mak,IT
影响因子：
6.5
作者：
M. Vanrollins;R. Murphy
通讯作者：
M. Vanrollins;R. Murphy
影响因子：
2.9
作者：
CATHCART, R;SCHWIERS, E;AMES, BN
通讯作者：
AMES, BN
缺氧、缺血和炎症。
DOI：
--
发表时间：
1986
期刊：
Acta physiologica Scandinavica. Supplementum
影响因子：
--
作者：
Weiss,SJ
通讯作者：
Weiss,SJ
DOI：
10.1073/pnas.79.3.767
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
CAPDEVILA, J;MARNETT, LJ;ESTABROOK, RW
通讯作者：
ESTABROOK, RW