Genetic diversity and relationships of two pathovars of Pseudomonas syringae.
Genetic diversity and relationships of two pathovars of Pseudomonas syringae.
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丁香假单胞菌两个致病变型的遗传多样性和关系。
DOI：
10.1099/00221287-134-7-1949
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Selander,RK
中科院分区：
文献类型：
--
作者：
Denny,TP;Gilmour,MN;Selander,RK
确定典型OFP菌株--丁香假单胞菌两个致病变种内部和之间的遗传关系。番茄(P.S.番茄)和部分菌株OFP。用三种方法鉴定了丁香叶枯病菌(P.S.syringae)。DNA-DNA杂交实验表明，菌株OFP。S、番茄和P.与AP分离株DNA的同源性分别为86-100%和37-47%。在严格的条件下进行测试。对放置17P的26个基因座编码的酶的电泳变异进行了分析。这些菌株的平均每个基因座的遗传多样性为0.076。六个月。紫丁香形成了第二组6种电泳型，每个座位的平均遗传多样性较高，为0·479。平均遗传距离P。S。明天从P。丁香链霉菌(D=0.94)对于单一物种的菌株来说出人意料地大。3个克隆的杂交探针的限制性片段长度多态(RFLP)分析表明，每一个克隆的DNA片段长度多态(RFLP)。S、番茄和P.美国丁香花是独一无二的。建立了一种量化菌株间RFLP差异的方法，聚类分析揭示了菌株间的亲缘关系。美国番茄，但不在P。S.syringae，这与基于酶多态性的序列相似。讨论了这些发现对细菌系统学和流行病学的影响。
To determine genetic relationships within and between two pathovars ofPseudomonas syringae, strains typical ofP. syringaepv.tomato (P. s. tomato)and selected strains ofP. syringaepv.syringae (P. s. syringae)were characterized by three methods. DNA-DNA hybridization experiments showed that strains ofP. s. tomatoandP. s. syringaewere, respectively, 86-100% and 37–47% homologous to DNA from aP. s. tomatoreference strain when tested under stringent conditions. An analysis of electrophoretic variation in enzymes encoded by 26 loci placed 17P. s. tomatostrains studied in a group of four electrophoretic types, and these strains had a mean genetic diversity per locus of 0·076. SixP. s. syringaestrains formed a second group of six electrophoretic types, which had a higher mean genetic diversity per locus of 0·479. The mean genetic distance separatingP. s. tomatofromP. s. syringae(D= 0·94) was unexpectedly large for strains of a single species. An analysis of restriction fragment length polymorphisms (RFLPs) with three cloned hybridization probes demonstrated that each of theP. s. tomatoandP. s. syringaestrains was unique. A method was developed to quantify the RFLP difference between pairs of strains, and cluster analysis revealed relationships amongP. s. tomato, but not amongP. s. syringae, that were similar to those based on enzyme polymorphisms. Implications of these findings for bacterial systematics and epidemiology are discussed.
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I科假单胞菌科
DOI：
--
发表时间：
1989
期刊：
影响因子：
--
作者：
N. Palleroni
通讯作者：
N. Palleroni
假单胞菌属的植物病原成员
影响因子：
3.2
作者：
M. Schroth;D. C. Hildebrand;M. Starr
通讯作者：
M. Starr
DOI：
10.1099/00207713-37-3-214
发表时间：
1987-07-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
LAZO, GR;ROFFEY, R;GABRIEL, DW
通讯作者：
GABRIEL, DW
DNA 同源矩阵和非随机变异概念作为植物病原菌和其他细菌分类学处理的基础
影响因子：
10.2
作者：
D. C. Hildebrand;A. Schroth;O. Huisman
通讯作者：
O. Huisman
通过铁载体相关基因探针的 Southern 杂交分析揭示荧光假单胞菌的基因型多样性
影响因子：
7.2
作者：
E. C. Lawson;C. Jonsson;B. Hemming
通讯作者：
B. Hemming