The effect of ultrasound treatment in combination with nisin on the inactivation of Listeria innocua and Escherichia coli.
The effect of ultrasound treatment in combination with nisin on the inactivation of Listeria innocua and Escherichia coli.
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DOI：
10.1016/j.ultsonch.2021.105776
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发表时间：
2021-11
影响因子：
8.4
通讯作者：
Bussemaker M
中科院分区：
文献类型：
--
作者：
Costello KM;Velliou E;Gutierrez-Merino J;Smet C;Kadri HE;Impe JFV;Bussemaker M
超声（US）和乳酸链球菌素用于微生物灭活的多频研究。US仅在500 kHz时影响大肠杆菌；innocua对所研究的所有频率都具有抗性。在US之前使用Nisin可以增强大肠杆菌的失活，而在US之后使用则没有。由于US使外膜不稳定，允许乳酸链球菌素渗透。体系结构（粘度）降低US失活效果。超声波，单独或与天然抗菌剂结合，是一种新的食品加工技术，可以取代传统的食品去污染方法，因为它比传统的灭菌（热处理）更温和，并保持理想的感官特性。然而，超声波的功效可能受到食物结构/成分以及应用联合治疗的顺序的影响。更具体地说，针对不同细胞成分的治疗如果按适当的顺序施用，可能会导致增强的失活。微生物特性（革兰氏阳性/革兰氏阴性）也会影响治疗效果。这项工作提出了一个系统的研究超声和乳酸链球菌素在一系列空化条件（44,500,1000 kHz）下对无性李斯特菌（革兰氏阳性）和大肠杆菌（革兰氏阴性）灭活的联合效应。采用不同的处理顺序，并通过改变黄原胶的浓度（0 ~ 0.5% w/v）来研究体系结构的影响。监测微生物失活动力学，并利用先进的显微镜和流式细胞术技术量化治疗对细胞水平的影响。超声波仅在500 kHz时对大肠杆菌有效，而innocul对所研究的所有频率都显示出抗性。在500 kHz的频率下，nisin和超声联合使用可增强大肠杆菌的失活，但仅限于在超声治疗前应用nisin。系统结构对失活效果有负面影响。超声波和乳酸链球菌素对大肠杆菌的联合作用归因于超声引起的外膜的短暂不稳定，使乳酸链球菌素穿透细胞质膜并促进细胞失活。
A multi-frequency study of ultrasound (US) and nisin for microbial inactivation. US impacts E. coli at 500 kHz only; L. innocua resists all frequencies studied. Nisin applied before US enhances inactivation of E. coli but not when applied after. Attributed to outer membrane destabilisation by US allowing nisin penetration. System structure (viscosity) reduces US inactivation efficacy. Ultrasound, alone or in combination with natural antimicrobials, is a novel food processing technology of interest to replace traditional food decontamination methods, as it is milder than classical sterilisation (heat treatment) and maintains desirable sensory characteristics. However, ultrasound efficacy can be affected by food structure/composition, as well as the order in which combined treatments are applied. More specifically, treatments which target different cell components could result in enhanced inactivation if applied in the appropriate order. The microbial properties i.e. Gram positive/Gram negative can also impact the treatment efficacy. This work presents a systematic study of the combined effect of ultrasound and nisin on the inactivation of the bacteria Listeria innocua (Gram positive) and Escherichia coli (Gram negative), at a range of cavitation conditions (44, 500, 1000 kHz). The order of treatment application was varied, and the impact of system structure was also investigated by varying the concentration of Xanthan gum used to create the food model systems (0 – 0.5% w/v). Microbial inactivation kinetics were monitored, and advanced microscopy and flow cytometry techniques were utilised to quantify the impact of treatment on a cellular level. Ultrasound was shown to be effective against E. coli at 500 kHz only, with L. innocua demonstrating resistance to all frequencies studied. Enhanced inactivation of E. coli was observed for the combination of nisin and ultrasound at 500 kHz, but only when nisin was applied before ultrasound treatment. The system structure negatively impacted the inactivation efficacy. The combined effect of ultrasound and nisin on E. coli was attributed to short-lived destabilisation of the outer membrane as a result of sonication, allowing nisin to penetrate the cytoplasmic membrane and facilitate cell inactivation.
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影响因子：
2.4
作者：
Collin, Jamie R. T.;Coussios, Constantin C.
通讯作者：
Coussios, Constantin C.
DOI：
10.3390/molecules26071890
发表时间：
2021-03-26
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
De la Ossa JG;El Kadri H;Gutierrez-Merino J;Wantock T;Harle T;Seggiani M;Danti S;Di Stefano R;Velliou E
通讯作者：
Velliou E
影响因子：
12.8
作者：
Foladori, Paola;Laura, Bruni;Giuliano, Ziglio
通讯作者：
Giuliano, Ziglio
影响因子：
2
作者：
Baumann, Adam R.;Martin, Scott E.;Hao Feng
通讯作者：
Hao Feng
影响因子：
4
作者：
Ananta, E;Voigt, D;Knorr, D
通讯作者：
Knorr, D