Combined Antimicrobial Effect of Bio-Waste Olive Leaf Extract and Remote Cold Atmospheric Plasma Effluent.
Combined Antimicrobial Effect of Bio-Waste Olive Leaf Extract and Remote Cold Atmospheric Plasma Effluent.
复制标题
DOI：
10.3390/molecules26071890
复制
发表时间：
2021-03-26
期刊：
影响因子：
--
通讯作者：
Velliou E
中科院分区：
文献类型：
--
作者：
De la Ossa JG;El Kadri H;Gutierrez-Merino J;Wantock T;Harle T;Seggiani M;Danti S;Di Stefano R;Velliou E
采用橄榄叶提取物（OLE）和冷常压等离子体（CAP）作为绿色抗菌处理的新策略。具体来说，我们报告了OLE + CAP联合使用对三种病原体的初步调查，选择代表医疗和食品行业（即大肠杆菌，金黄色葡萄球菌和innocua乳杆菌）。结果表明，100 mg/mL（总多酚）浓度的OLE具有一定的抑菌活性，但仍不足以使细菌完全失活。通过使用普通OLE，我们可以显著降低革兰氏阳性金黄色葡萄球菌和innocul的生长，但对革兰氏阴性大肠杆菌没有影响。相反，我们证明了OLE + CAP在大肠杆菌、金黄色葡萄球菌和无害乳杆菌样品中6小时后具有显著的去污效果，这种效果在金黄色葡萄球菌菌株中可保持24小时。24 h后，E. coli和L. innocua再次生长。在后者中，单独使用OLE对细菌生长最有效。通过进一步调整OLE + CAP技术的参数，例如OLE量和CAP暴露量，可以延长初始强力去污的时间。由于OLE来源于生物废物，而CAP是一种基于电离空气的非热技术，因此我们提出OLE + CAP作为细菌净化的潜在绿色平台。作为组合，OLE和CAP可以产生比单独更好的抗菌活性，并且可以取代或补充食品和生物医学行业的传统热程序。
A novel strategy involving Olive Leaf Extract (OLE) and Cold Atmospheric Plasma (CAP) was developed as a green antimicrobial treatment. Specifically, we reported a preliminary investigation on the combined use of OLE + CAP against three pathogens, chosen to represent medical and food industries (i.e., E. coli, S. aureus and L. innocua). The results indicated that a concentration of 100 mg/mL (total polyphenols) in OLE can exert an antimicrobial activity, but still insufficient for a total bacterial inactivation. By using plain OLE, we significantly reduced the growth of Gram positive S. aureus and L. innocua, but not Gram-negative E. coli. Instead, we demonstrated a remarkable decontamination effect of OLE + CAP in E. coli, S. aureus and L. innocua samples after 6 h. This effect was optimally maintained up to 24 h in S. aureus strain. E. coli and L. innocua grew again in 24 h. In the latter strain, OLE alone was most effective to significantly reduce bacterial growth. By further adjusting the parameters of OLE + CAP technology, e.g., OLE amount and CAP exposure, it could be possible to prolong the initial powerful decontamination over a longer time. Since OLE derives from a bio-waste and CAP is a non-thermal technology based on ionized air, we propose OLE + CAP as a potential green platform for bacterial decontamination. As a combination, OLE and CAP can lead to better antimicrobial activity than individually and may replace or complement conventional thermal procedures in food and biomedical industries.
登录
查看更多内容
影响因子：
2.1
作者：
Danti, Serena;Azimi, Bahareh;Berrettini, Stefano
通讯作者：
Berrettini, Stefano
DOI：
10.1016/j.jtcme.2017.10.006
发表时间：
2019-10-01
影响因子：
4.5
作者：
Amaral, Guilherme Pires;Mizdal, Caren Rigon;Fachinetto, Roselei
通讯作者：
Fachinetto, Roselei
DOI：
10.3390/foods9091336
发表时间：
2020-09-22
期刊：
Foods (Basel, Switzerland)
影响因子：
--
作者：
Djenane D;Aboudaou M;Djenane F;García-Gonzalo D;Pagán R
通讯作者：
Pagán R
影响因子：
5.2
作者：
Djenane, Djamel;Gomez, Diego;Arino, Agustin
通讯作者：
Arino, Agustin
DOI：
10.1177/2156587217700772
发表时间：
2017-10
期刊：
Journal of evidence-based complementary & alternative medicine
影响因子：
--
作者：
Fani M;Kohanteb J
通讯作者：
Kohanteb J