Topographical organization of the rat suprachiasmatic‐paraventriocular projection
Topographical organization of the rat suprachiasmatic‐paraventriocular projection
复制标题
大鼠视交叉上-心室旁投影的地形组织
DOI：
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
J. Mikkelsen
中科院分区：
文献类型：
--
作者：
N. Vrang;P. J. Larsen;M. Møller;J. Mikkelsen
视交叉上核(SCN)是参与哺乳动物昼夜节律产生的主要起搏器。令人惊讶的是，许多节律的表达似乎是通过起搏器有限的传出投射系统来调节的，其中最大的通路终止于室下区和下丘脑室旁核。为了研究SCN这一主要流出通路的可能的局部组织结构，将顺行示踪剂菜豆亮氨酸凝集素(PHA-L)或逆行示踪剂霍乱毒素B亚单位(ChB)分别集中在SCN的不同亚部(PHA-L)或室旁核(ChB)。将PHA-L注射到整个SCN，不仅显示了一个主要投射到室旁区域，而且还投射到了室旁核的内侧和背侧小细胞亚核。与涉及整个核团的注射不同，以背内侧核为中心注射PHA-L可在下丘脑室旁核区的小细胞亚区产生较多的PHA-L免疫反应阳性纤维，而室旁下区的终末野则较少。ChB逆行示踪还显示了SCN传出系统的形态。在下丘脑室旁核背侧部注射ChB，不影响下丘脑室旁下区，在SCN的背内侧部产生大量逆行标记细胞。相反，在室旁区域注射ChB会导致标记聚集在SCN更腹外侧侧的神经元。目前的数据为大鼠SCN的主要传出、投射系统的地形图提供了证据，暗示大鼠SCN的不同亚部分可能含有部分不同的潜在神经递质物质，可能调节不同的昼夜节律。©1995 Wiley-Liss公司
The suprachiasmatic nucleus (SCN) is a dominant pacemaker involved in the generation of circadian rhythms in mammals. Surprisingly, the expression of the many rhythms appears to be mediated via a limited efferent projection system of the pacemaker, of which the largest pathway terminates in the subparaventricular area and in the paraventricular nucleus of the hypothalamus. In order to investigate a possible topographical organization of this major outflow pathway of the SCN, microiontophoretic injections of the anterograde tracer Phaseolus vulgaris‐leucoagglutinin (PHA‐L) or the retrograde tracer cholera toxin subunit B (ChB) were centered in distinct subparts of the SCN (PHA‐L) or in the subparaventricular areaparaventricular nucleus (ChB), respectively. PHA‐L injections involving the entire SCN revealed not only a major projection to the subparaventricular area, but also one directed towards the medial and dorsal parvicellular subnuclei of the paraventricular nucleus. As opposed to injections involving the entire nucleus, injections of PHA‐L centered in the dorsomedial subdivision of the SCN resulted in a relatively larger number of PHA‐Limmunoreactive fibers in the parvicellular subdivisions of the PVN, whereas the terminal field in the subparaventricular area was less substantial. A topography of the SCN efferent output system was also revealed by the retrograde tracing with ChB. Injections of ChB in the dorsal part of the paraventricular hypothalamic nucleus, not involving the underlying subparaventricular area, gave rise to a population of retrogradely labeled cells in the dorsomedial part of the SCN. In contrast, ChB injections in the subparaventricular area resulted in labeling of neurons clustered in a more ventrolateral aspect of the SCN. The present data provide evidence for a topography in the major efferent, projection system from the SCN, implying that different subparts of the rat SCN, presumably containing partly different potential neurotransmitter substances, may regulate different circadian rhythms. © 1995 Wiley‐Liss, Inc.
通过原位杂交测量两性大鼠下丘脑室旁核前促肾上腺皮质激素释放激素信使核糖核酸含量的昼夜变化。
影响因子：
4.8
作者：
Watts,AG;Swanson,LW
通讯作者：
Swanson,LW
视交叉上核中的加压素 mRNA：聚腺苷酸尾长的日常调节。
DOI：
10.1126/science.3388044
发表时间：
1988
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Robinson,BG;Frim,DM;Schwartz,WJ;Majzoub,JA
通讯作者：
Majzoub,JA