Strikingly bacteria-like and gene-rich mitochondrial genomes throughout jakobid protists.
Strikingly bacteria-like and gene-rich mitochondrial genomes throughout jakobid protists.
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DOI：
10.1093/gbe/evt008
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发表时间：
2013
影响因子：
3.3
通讯作者：
Lang BF
中科院分区：
文献类型：
--
作者：
Burger G;Gray MW;Forget L;Lang BF
已知最像细菌的线粒体基因组是雅各布鞭毛虫ReclinomonasamericanaNZ。该基因组还编码线粒体DNA（mtDNA）中最大的已知基因组，包括RNase P（转移RNA加工）的RNA亚基，一种还原形式的转移信使RNA（翻译控制）和四亚基细菌样RNA聚合酶，在其他真核生物中被细胞核编码的单亚基噬菌体样酶取代。此外，蛋白质编码基因之前是潜在的Shine-Dalgarno翻译起始基序。是否相似的祖先线粒体特征也存在于R.美国人不知道在这里，我们报告了9个mtDNA的比较分析，从五个遥远的jakobid属：安达卢西亚，Histiona，Jakoba，Reclinomonas，和Seculamonas。我们发现安达卢西亚的线粒体基因组比R. americana NZ额外的基因是rpl 35（一种大亚基线粒体蛋白）和cox 15（参与细胞色素氧化酶组装），它们是其他真核生物的细胞核编码。Andalucia cox 15与自由生活的α-变形杆菌Tistrella mobilis中的同源物惊人地相似。同样，雅各布虫mtDNA中一个长的、高度保守的基因簇，是原核操纵子的明显痕迹，显示出与自由生活的α-变形菌比立克次体物种更接近的基因顺序。虽然jakobid mtDNA总体上具有细菌样特征，但它们也显示出一些显著不同的特征，例如厄瓜多尔Seculamonas ecuadoriensis的3′-tRNA编辑和Jakoba libera的基因组线性化。线粒体DNA编码的蛋白质的系统发育分析强烈支持单系的jakobids与Andalucia作为最深的分歧。然而，目前还不清楚哪一个α-变形菌群是最接近的线粒体亲属。
The most bacteria-like mitochondrial genome known is that of the jakobid flagellate Reclinomonas americana NZ. This genome also encodes the largest known gene set among mitochondrial DNAs (mtDNAs), including the RNA subunit of RNase P (transfer RNA processing), a reduced form of transfer–messenger RNA (translational control), and a four-subunit bacteria-like RNA polymerase, which in other eukaryotes is substituted by a nucleus-encoded, single-subunit, phage-like enzyme. Further, protein-coding genes are preceded by potential Shine–Dalgarno translation initiation motifs. Whether similarly ancestral mitochondrial characters also exist in relatives of R. americana NZ is unknown. Here, we report a comparative analysis of nine mtDNAs from five distant jakobid genera: Andalucia, Histiona, Jakoba, Reclinomonas, and Seculamonas. We find that Andalucia godoyi has an even larger mtDNA gene complement than R. americana NZ. The extra genes are rpl35 (a large subunit mitoribosomal protein) and cox15 (involved in cytochrome oxidase assembly), which are nucleus encoded throughout other eukaryotes. Andalucia cox15 is strikingly similar to its homolog in the free-living α-proteobacterium Tistrella mobilis. Similarly, a long, highly conserved gene cluster in jakobid mtDNAs, which is a clear vestige of prokaryotic operons, displays a gene order more closely resembling that in free-living α-proteobacteria than in Rickettsiales species. Although jakobid mtDNAs, overall, are characterized by bacteria-like features, they also display a few remarkably divergent characters, such as 3′-tRNA editing in Seculamonas ecuadoriensis and genome linearization in Jakoba libera. Phylogenetic analysis with mtDNA-encoded proteins strongly supports monophyly of jakobids with Andalucia as the deepest divergence. However, it remains unclear which α-proteobacterial group is the closest mitochondrial relative.
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影响因子：
2.2
作者：
FLAVIN, M;NERAD, TA
通讯作者：
NERAD, TA
影响因子：
3.9
作者：
Cermakian, N;Ikeda, TM;Cedergren, R
通讯作者：
Cedergren, R
影响因子：
14.9
作者：
Cermakian, N;Ikeda, TM;Gray, MW
通讯作者：
Gray, MW
DOI：
10.1099/mic.0.038646-0
发表时间：
2010-07
期刊：
Microbiology (Reading, England)
影响因子：
--
作者：
Fricova D;Valach M;Farkas Z;Pfeiffer I;Kucsera J;Tomaska L;Nosek J
通讯作者：
Nosek J
影响因子：
10.7
作者：
Edgcomb, VP;Roger, AJ;Sogin, ML
通讯作者：
Sogin, ML