Characterization of the soybean genome using EST-derived microsatellite markers.
Characterization of the soybean genome using EST-derived microsatellite markers.
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DOI：
10.1093/dnares/dsm025
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发表时间：
2007-12-31
期刊：
影响因子：
--
通讯作者：
Tabata S
中科院分区：
文献类型：
--
作者：
Hisano H;Sato S;Isobe S;Sasamoto S;Wada T;Matsuno A;Fujishiro T;Yamada M;Nakayama S;Nakamura Y;Watanabe S;Harada K;Tabata S
我们利用表达序列标签（EST）衍生的微卫星标记构建了大豆的高密度遗传连锁图谱。共设计了6920对引物，对63676条公开的非冗余大豆EST序列进行扩增，扩增率为10.9%。采用10%聚丙烯酰胺凝胶电泳技术，对日本品种‘三铃大津’和中国品系‘磨石豆公503’两个亲本植株的多态性进行了研究。显示多态性的引物对，然后用于基因分型94重组自交系（RILs）来自父母之间的杂交。除了以前报道的标记，680 EST衍生的微卫星标记进行了连锁分析。结果表明，935个标记位点被成功定位到20个连锁群上，总长度为2700.3cM，其中668个EST来源的微卫星标记检测到693个位点，105个RFLP标记、136个基因组来源的微卫星标记和1个表型标记检测到242个位点。利用668个EST微卫星标记（对应686个标记位点）分析了23个大豆品种/品系和1个野生大豆品系的等位基因变异，以确定这些标记在大豆种质资源中的可转移性。在大豆和模式豆科植物百脉根的基因组之间的节段水平上观察到有限程度的宏同线性，这表明在物种分离后和古多倍体大豆基因组建立期间发生了相当大的基因组改组。
We generated a high-density genetic linkage map of soybean using expressed sequence tag (EST)-derived microsatellite markers. A total of 6920 primer pairs (10.9%) were designed to amplify simple sequence repeats (SSRs) from 63 676 publicly available non-redundant soybean ESTs. The polymorphism of two parent plants, the Japanese cultivar ‘Misuzudaizu’ and the Chinese line ‘Moshidou Gong 503’, were examined using 10% polyacrylamide gel electrophoresis. Primer pairs showing polymorphism were then used for genotyping 94 recombinant inbred lines (RILs) derived from a cross between the parents. In addition to previously reported markers, 680 EST-derived microsatellite markers were selected and subjected to linkage analysis. As a result, 935 marker loci were mapped successfully onto 20 linkage groups, which totaled 2700.3 cM in length; 693 loci were detected using the 668 EST-derived microsatellite markers developed in this study, the other 242 loci were detected with 105 RFLP markers, 136 genome-derived microsatellite markers, and one phenotypic marker. We examined allelic variation among 23 soybean cultivars/lines and a wild soybean line using 668 mapped EST-derived microsatellite markers (corresponding to 686 marker loci), in order to determine the transferability of the markers among soybean germplasms. A limited degree of macrosynteny was observed at the segmental level between the genomes of soybean and the model legume Lotus japonicus, which suggests that considerable genome shuffling occurred after separation of the species and during establishment of the paleopolyploid soybean genome.
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DOI：
10.1073/pnas.0402251101
发表时间：
2004-10-26
影响因子：
11.1
作者：
Choi, HK;Mun, JH;Cook, DR
通讯作者：
Cook, DR
影响因子：
7.4
作者：
Hall, AE;Fiebig, A;Preuss, D
通讯作者：
Preuss, D
影响因子：
5.4
作者：
KEIM, P;BEAVIS, W;FREESTONE, R
通讯作者：
FREESTONE, R
影响因子：
3.1
作者：
MORGANTE, M;RAFALSKI, A;OLIVIERI, AM
通讯作者：
OLIVIERI, AM
影响因子：
4.1
作者：
Hossain, KG;Kawai, H;Harada, K
通讯作者：
Harada, K