Overexpression of the Capebp2 Gene Encoding the PEBP-like Protein Promotes the Cap Redifferentiation in Cyclocybe aegerita.
Overexpression of the Capebp2 Gene Encoding the PEBP-like Protein Promotes the Cap Redifferentiation in Cyclocybe aegerita.
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DOI：
10.3390/jof9060657
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发表时间：
2023-06-12
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磷脂酰乙醇胺结合蛋白（PEBP）广泛参与多种生理行为，如植物从营养生长到生殖生长的转变、人类肿瘤发生等。然而，很少有功能研究探讨PEBP基因影响真菌发育的功能。本研究根据基因组序列和基因预测，从Cyclocybe aegerita AC0007菌株中克隆了Capebp2，并将CaPEBP2与植物、动物、真菌和细菌等其他生物来源的其他PEBP蛋白进行序列比对，表明PEBP在真菌中具有较低的序列相似性，而所有蛋白序列均具有一些保守基序，例如DPDAP和HRY。表达分析显示，与菌丝体相比，子实体中Capebp2的转录水平增加了约20倍。为了揭示Capebp2在C. aegetita发育中的功能，将Capebp2克隆到由肌动蛋白启动子驱动的pATH载体中以获得过表达转化株系。子实体实验表明，过表达Capebp2的转化菌株在子实体发育阶段，表面出现菌盖再分化，包括完整的子实体或部分片片，纵切面显示再生体或片片全部从果肉中萌发，并与母子实体共享表皮。综上所述，本研究记录了Capebp2的序列特征、不同发育阶段的表达水平以及对子实体发育的功能，为研究pebp在担子菌发育过程中的作用提供了参考。重要的是，pebp 的基因挖掘、功能表征以及所涉及的调节途径需要在进一步的研究中揭示。
Phosphatidylethanolamine-binding protein (PEBP) is widely involved in various physiological behaviors, such as the transition from vegetative growth to reproductive growth in plants, tumorigenesis in the human, etc. However, few functional studies have examined pebp genes affecting the development of fungi. In this study, Capebp2 was cloned from Cyclocybe aegerita AC0007 strains based on the genome sequence and gene prediction, and the sequence alignment of CaPEBP2 with other PEBP proteins from other biological sources including plant, animal, fungi, and bacteria indicated that PEBP had low sequence similarity in fungi, whereas all protein sequences had some conserved motifs such as DPDAP and HRY. Expression analysis showed the transcription level of Capebp2 increased approximately 20-fold in fruiting bodies compared with mycelia. To uncover the function of Capebp2 in C. aegetita development, Capebp2 was cloned into a pATH vector driven by the actin promoter for obtaining overexpression transformant lines. Fruiting experiments showed the transformed strains overexpressing Capebp2 exhibited redifferentiation of the cap on their surface, including intact fruiting bodies or partial lamella during fruiting development stage, and the longitudinal section indicated that all regenerated bodies or lamella sprouted from the flesh and shared the epidermis with the mother fruiting bodies. In summary, the sequence characterization of Capebp2, expression level during different development stages, and function on fruiting body development were documented in this study, and these findings provided a reference to study the role of pebp in the development process of basidiomycetes. Importantly, gene mining of pebp, function characterization, and the regulating pathways involved need to be uncovered in further studies.
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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通讯作者：
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发表时间：
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期刊：
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影响因子：
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