A multiplex lateral flow immunoassay for the rapid identification of NDM-, KPC-, IMP- and VIM-type and OXA-48-like carbapenemase-producing Enterobacteriaceae.
A multiplex lateral flow immunoassay for the rapid identification of NDM-, KPC-, IMP- and VIM-type and OXA-48-like carbapenemase-producing Enterobacteriaceae.
复制标题
DOI：
10.1093/jac/dkx521
复制
发表时间：
2018-04-01
期刊：
影响因子：
--
通讯作者：
Volland H
中科院分区：
文献类型：
--
作者：
Boutal H;Vogel A;Bernabeu S;Devilliers K;Creton E;Cotellon G;Plaisance M;Oueslati S;Dortet L;Jousset A;Simon S;Naas T;Volland H
The global spread of carbapenemase-producing Enterobacteriaceae represents a substantial challenge in clinical practice and rapid and reliable detection of these organisms is essential. The aim of this study was to develop and validate a lateral flow immunoassay (Carba5) for the detection of the five main carbapenemases (KPC-, NDM-, VIM- and IMP-type and OXA-48-like). Carba5 was retrospectively and prospectively evaluated using 296 enterobacterial isolates from agar culture. An isolated colony was suspended in extraction buffer and then loaded on the manufactured Carba5. All 185 isolates expressing a carbapenemase related to one of the Carba5 targets were correctly and unambiguously detected in <15 min. All other isolates gave negative results except those producing OXA-163 and OXA-405, which are considered low-activity carbapenemases. No cross-reaction was observed with non-targeted carbapenemases, ESBLs, AmpCs or oxacillinases (OXA-1, -2, -9 and -10). Overall, this assay reached 100% sensitivity and 95.3% (retrospectively) to 100% (prospectively) specificity. Carba5 is efficient, rapid and easy to implement in the routine workflow of a clinical microbiology laboratory for confirmation of the five main carbapenemases encountered in Enterobacteriaceae.
登录
查看更多内容
影响因子：
9.4
作者：
Lasserre, Camille;De Saint Martin, Luc;Tande, Didier
通讯作者：
Tande, Didier
实时多路复用PCR的多中心评估用于检测碳青霉酶基因OXA-48，VIM，IMP，NDM和KPC。
影响因子：
3.7
作者：
van der Zee A;Roorda L;Bosman G;Fluit AC;Hermans M;Smits PH;van der Zanden AG;Te Witt R;Bruijnesteijn van Coppenraet LE;Cohen Stuart J;Ossewaarde JM
通讯作者：
Ossewaarde JM
影响因子：
9.4
作者：
Tato M;Ruiz-Garbajosa P;Traczewski M;Dodgson A;McEwan A;Humphries R;Hindler J;Veltman J;Wang H;Cantón R
通讯作者：
Cantón R
影响因子：
3.2
作者：
Doi Y;Paterson DL
通讯作者：
Paterson DL
影响因子：
5.2
作者：
Glupczynski, Youri;Evrard, Stephanie;Bogaerts, Pierre
通讯作者：
Bogaerts, Pierre