RNA-Seq reveals a xenobiotic stress response in the soybean aphid, Aphis glycines, when fed aphid-resistant soybean.
RNA-Seq reveals a xenobiotic stress response in the soybean aphid, Aphis glycines, when fed aphid-resistant soybean.
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DOI：
10.1186/1471-2164-15-972
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发表时间：
2014-11-16
期刊：
影响因子：
4.4
通讯作者：
Michel AP
中科院分区：
文献类型：
--
作者：
Bansal R;Mian MA;Mittapalli O;Michel AP
虽然最近的许多研究扩大了我们对蚜虫与其寄主植物之间的分子相互作用的理解，但对于大豆蚜虫来说，这方面的研究还很少。自北美入侵以来，大豆已成为这一重要作物上最具破坏性的害虫之一。目前已鉴定出5个与大豆寄主植物抗性有关的基因，但大豆种群已经适应了这些抗性基因。了解抗性大豆和大豆农杆菌之间的分子相互作用可以为了解其适应机制提供线索。在这里，我们使用RNA测序来比较和对比大豆抗性(Rag1)和感病大豆(Rag1)的甘氨酸基因表达。结合先前甘氨酸转录组的结果，我们产生了64,860个高质量的转录本，总计41,151,086个碱基。统计分析发现了914个差异表达显著的基因。在抗性植株(N = 35 2)上，甘氨酸高表达的基因大多与胁迫和解毒有关，如细胞色素P450、谷胱甘肽S转移酶、羧酸酯酶和ABC转运蛋白。在抗性植株上，共有562个基因的转录本丰度较低。从我们广泛的转录组数据中，我们还发现了唾液效应蛋白(N = 73)的编码基因。其中6个效应基因在取食抗性大豆的大豆赤霉菌中具有较低的转录丰度。总体而言，甘氨酸呈现出异种挑战的典型模式，从而验证了Rag1中的抗生素，推测是通过有毒的次生代谢物介导的。此外，本研究还鉴定了许多甘氨酸基因和基因家族，这些基因和基因家族处于大豆分子相互作用的前沿。在其他生物型中对这些基因的进一步研究可能会揭示出对抗性植物的适应机制。本文的在线版本(DOI：10.1186/1471-2164-15-972)包含补充材料，可供授权用户使用。
While much recent research has expanded our understanding of the molecular interactions between aphids and their host plants, it is lacking for the soybean aphid, Aphis glycines. Since its North American invasion, A. glycines has become one of the most damaging insect pests on this important crop. Five soybean genes for host plant resistance to A. glycines have been identified, but populations of A. glycines have already adapted to overcome these resistance genes. Understanding the molecular interactions between resistant soybean and A. glycines can provide clues to its adaptation mechanisms. Here, we used RNA-Sequencing to compare and contrast A. glycines gene expression when fed resistant (Rag1) and susceptible soybean. Combining results from a previous A. glycines transcriptome, we generated 64,860 high quality transcripts, totaling 41,151,086 bases. Statistical analysis revealed 914 genes with significant differential expression. Most genes with higher expression in A. glycines on resistant plants (N = 352) were related to stress and detoxification such as cytochrome P450s, glutathione-S-transferases, carboxyesterases, and ABC transporters. A total of 562 genes showed lower transcript abundance in A. glycines on resistant plants. From our extensive transcriptome data, we also identified genes encoding for putative salivary effector proteins (N = 73). Among these, 6 effector genes have lower transcript abundance in A. glycines feeding on resistant soybean. Overall, A. glycines exhibited a pattern typical of xenobiotic challenge, thereby validating antibiosis in Rag1, presumably mediated through toxic secondary metabolites. Additionally, this study identified many A. glycines genes and gene families at the forefront of its molecular interaction with soybean. Further investigation of these genes in other biotypes may reveal adaptation mechanisms to resistant plants. The online version of this article (doi:10.1186/1471-2164-15-972) contains supplementary material, which is available to authorized users.
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影响因子：
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