Transcriptomics of the bed bug (Cimex lectularius).
Transcriptomics of the bed bug (Cimex lectularius).
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DOI：
10.1371/journal.pone.0016336
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发表时间：
2011-01-19
期刊：
影响因子：
3.7
通讯作者：
Mittapalli O
中科院分区：
文献类型：
--
作者：
Bai X;Mamidala P;Rajarapu SP;Jones SC;Mittapalli O
臭虫（Cimex lectularius）是一种吸血昆虫，即将成为美国家庭中的主要害虫之一。蛭状芽孢杆菌对杀虫剂/农药的耐药性被认为是其突然死灰复燃的一个因素。尽管它的高影响地位，缺乏知识存在于基因组水平的C. lectularius。因此，我们对C. lectularius转录组进行了454焦磷酸测序，以确定与农药抗性有关的潜在基因。通过454焦磷酸测序，我们获得了216,419个reads， 79,596,412 bp，这些reads被组装成35,646个表达的序列标签（3902个contigs和31744个singleton）。近85.9%的褐螺旋藻序列与昆虫序列相似，44.8%的褐螺旋藻蛋白质与GenBank非冗余数据库序列不相似。KEGG分析揭示了参与农药抗性的几种解毒途径的假定成员。Lamprin结构域、Protein Kinase结构域、Protein Tyrosine Kinase结构域和cytochrome P450结构域是预测的C. lectularius序列的顶级Pfam结构域。初步评估推测的防御基因，包括细胞色素P450和谷胱甘肽-s -转移酶（GST），显示细胞色素P450 （CYP9）在农药暴露和农药敏感的C. lectularius群体中有较高的转录水平。在C. lectularius序列中预测到大量的单核苷酸多态性（296个）和微卫星位点（370个）。此外，从数据库中检索到59个沃尔巴克氏体推定序列。据我们所知，这是第一次阐明C. lectularius基因组成的研究。这项焦磷酸测序的工作为确定与C. lectularius农药抗性相关的潜在解毒基因提供了线索，并为未来的功能基因组学研究奠定了基础。
Bed bugs (Cimex lectularius) are blood-feeding insects poised to become one of the major pests in households throughout the United States. Resistance of C. lectularius to insecticides/pesticides is one factor thought to be involved in its sudden resurgence. Despite its high-impact status, scant knowledge exists at the genomic level for C. lectularius. Hence, we subjected the C. lectularius transcriptome to 454 pyrosequencing in order to identify potential genes involved in pesticide resistance. Using 454 pyrosequencing, we obtained a total of 216,419 reads with 79,596,412 bp, which were assembled into 35,646 expressed sequence tags (3902 contigs and 31744 singletons). Nearly 85.9% of the C. lectularius sequences showed similarity to insect sequences, but 44.8% of the deduced proteins of C. lectularius did not show similarity with sequences in the GenBank non-redundant database. KEGG analysis revealed putative members of several detoxification pathways involved in pesticide resistance. Lamprin domains, Protein Kinase domains, Protein Tyrosine Kinase domains and cytochrome P450 domains were among the top Pfam domains predicted for the C. lectularius sequences. An initial assessment of putative defense genes, including a cytochrome P450 and a glutathione-S-transferase (GST), revealed high transcript levels for the cytochrome P450 (CYP9) in pesticide-exposed versus pesticide-susceptible C. lectularius populations. A significant number of single nucleotide polymorphisms (296) and microsatellite loci (370) were predicted in the C. lectularius sequences. Furthermore, 59 putative sequences of Wolbachia were retrieved from the database. To our knowledge this is the first study to elucidate the genetic makeup of C. lectularius. This pyrosequencing effort provides clues to the identification of potential detoxification genes involved in pesticide resistance of C. lectularius and lays the foundation for future functional genomics studies.
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影响因子：
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通讯作者：
Barbehenn, RV
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