Virulence factors and o-serogroups profiles of uropathogenic Escherichia coli isolated from Iranian pediatric patients.
Virulence factors and o-serogroups profiles of uropathogenic Escherichia coli isolated from Iranian pediatric patients.
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DOI：
10.5812/ircmj.14627
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发表时间：
2014-02
影响因子：
0.4
通讯作者：
Khodaverdi Darian E
中科院分区：
文献类型：
--
作者：
Dormanesh B;Safarpoor Dehkordi F;Hosseini S;Momtaz H;Mirnejad R;Hoseini MJ;Yahaghi E;Tarhriz V;Khodaverdi Darian E
尿路致病性大肠杆菌 O- 血清群及其毒力因子是尿路感染最常见的原因。本研究的目的是研究从伊朗儿科患者中分离出的 UPEC 的毒力因子和 O 血清群谱。这项横断面调查是对从伊朗德黑兰 Baqiyatallah 医院儿科住院的 100 份尿液样本进行的。收集中流尿液以减少潜在的细菌、细胞和人为污染。所有样本均进行培养，阳性结果进行聚合酶链式反应检测pap、cnf1、afa、sfa和hlyA基因以及各种O血清群。我们发现 37.5% 的男孩和 75% 的女孩的大肠杆菌检测呈阳性。我们还发现 O1 (19.33%)、O2 (13.33%)、O6 (13.33%)、O4 (11.66%) 和 O18 (11.66%) 是最常检测到的血清群。总共有5%的菌株未检测到血清群。此外，所有这些 O 血清群均为 pap+、cnf1+、hlyA+ 和 afa+。总的来说，pap (70%)、cnf1 (56.66%) 和 hlyA (43.33%) 是两个研究儿童组中最常检测到的毒力基因。 sfa (30%) 和 afa (26.66%) 基因的发生率最低。应对伊朗儿科患者的尿路感染管理进行特殊的卫生保健。应进行更广泛的研究来评估其他 O 血清群与毒力基因之间的关系。
Uropathogenic Escherichia coli O- Serogroups with their virulence factors are the most prevalent causes of UTIs. The present investigation was performed to study the virulence factors and O-Serogroups profiles of UPEC isolated from Iranian pediatric patients. This cross sectional investigation was performed on 100 urine samples collected from hospitalized pediatrics of Baqiyatallah Hospital, Tehran, Iran. Midstream urine was collected to decrease potential bacterial, cellular and artifactual contamination. All samples were cultured and those with positive results were subjected to polymerase chain reactions to detect pap, cnf1, afa, sfa and hlyA genes and various O- Serogroups. We found that 37.5% of boys and 75% of girls had positive results for Escherichia coli. We also found that O1 (19.33%), O2 (13.33%), O6 (13.33%), O4 (11.66%), and O18 (11.66 %) were the most commonly detected Serogroups. Totally, the serogroup of 5% of all strains were not detected. In addition, all of these O- Serogroups were pap+, cnf1+, hlyA+, and afa+. Totally, pap (70 %), cnf1 (56.66 %), and hlyA (43.33 %) were the most commonly detected virulence genes in the both studied groups of children. The sfa (30 %) and afa (26.66 %) genes had the lowest incidence rates. Special health care should be performed on UTIs management in Iranian pediatric patients. Extended researches should be performed to evaluate relation between other O-Serogroups and virulent genes.
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影响因子：
4
作者：
Foxman, B
通讯作者：
Foxman, B
DOI：
10.1152/ajplung.00482.2004
发表时间：
2005-08-01
影响因子：
4.9
作者：
Russo, TA;Davidson, BA;Knight, PR
通讯作者：
Knight, PR
影响因子：
3.1
作者：
Hagan, Erin C.;Mobley, Harry L. T.
通讯作者：
Mobley, Harry L. T.
影响因子：
6.4
作者：
Bauer, RJ;Zhang, LX;Marrs, CF
通讯作者：
Marrs, CF
影响因子：
--
作者：
Abe, Cecilia M.;Salvador, Fabia A.;Gomes, Tania A. T.
通讯作者：
Gomes, Tania A. T.