Delayed egg hatching of Anopheles gambiae (Diptera: Culicidae) pending water agitation.
Delayed egg hatching of Anopheles gambiae (Diptera: Culicidae) pending water agitation.
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DOI：
10.1603/me13100
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发表时间：
2014-05
影响因子：
2.1
通讯作者：
Foster WA
中科院分区：
文献类型：
--
作者：
Ebrahimi B;Shakibi S;Foster WA
产卵在水面上的蚊卵通常在里面的胚胎完全形成一龄幼虫后不久就会自发孵化。然而，我们发现冈比亚按蚊，非洲疟疾的重要媒介，表现出延迟孵化，直到水面搅动，这是大多数实验室殖民地被忽视的特征。在胚胎发育完成前的24小时内进行搅动，不能在第二天(第2天)刺激延迟的胚胎后孵化，此时1%的卵已经自发孵化。然而，在第2天，这些休眠的一龄幼虫在水中搅拌5min后，几乎立即孵化出63.3%的幼虫，而未搅拌的对照幼虫的孵化率为0%，在随后的24小时内，这些幼虫的孵化率分别为3.9%和0.3%。每日搅拌7d后的平均累计孵化率为83.1%，而未搅拌的孵化率为1.1%。卵成组试验表明，卵密度和已孵化幼虫的活跃度均无刺激作用。卵在25.5℃或15.5℃下贮藏1-4wk，然后在25.5℃下每天搅拌6d，活力逐渐下降。在较低的温度下，活力维持的时间较长。搅动诱导的卵孵化对东亚按蚊雨季和旱季生态的影响。对冈比亚亚纲进行了讨论。暂停孵化和冷藏已被证明是高效大规模饲养和准确模拟基于天气的种群动态的便利条件。
Mosquito eggs laid on water surfaces typically hatch spontaneously soon after the embryos within them become fully formed first-instar larvae. However, we have found that Anopheles gambiae Giles, an important vector of malaria in Africa, exhibits delayed hatching until the water surface is agitated, a feature overlooked in most laboratory colonies. Agitation within 24 h postoviposition, before embryonation was complete, failed to stimulate delayed postembryonic hatching of isolated eggs on the following day (day 2), when <1% had hatched spontaneously. However, 5 min of water agitation of these dormant pharate first-instar larvae on day 2 resulted in an almost immediate hatch of 63.3 versus 0% of nonagitated controls, plus another 3.9 versus 0.3%, respectively, during the following 24 h. With daily agitation, installment hatching occurred mainly during 2– 6 d postoviposition. The mean cumulative hatch after 7 d of daily agitation was 83.1 versus 1.1% of nonagitated eggs. Experiments with eggs in groups demonstrated that egg density and activity of already-hatched larvae had no stimulatory effect. Eggs stored 1–4 wk at 25.5 or at 15.5°C, and then agitated daily for 6 d at 25.5°C, showed a gradual decline in viability. Viability was sustained longer at the lower temperature. Implications of agitation-induced egg hatching for rainy-season and dry-season ecology of An. gambiae are discussed. Suspended hatching and cool storage already are proving convenient for efficient mass rearing and accurate modeling of weather-based population dynamics.
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影响因子：
1.9
作者：
Roberts, DM
通讯作者：
Roberts, DM
影响因子：
--
作者：
Miller, James R.;Huang, Juan;Walker, Edward D.
通讯作者：
Walker, Edward D.
DOI：
10.2987/8756-971x(2007)23
发表时间：
2007-01-01
影响因子：
1
作者：
Platzer, Edward G. -
通讯作者：
Platzer, Edward G. -
影响因子：
2.3
作者：
SHROYER, DA;CRAIG, GB
通讯作者：
CRAIG, GB
中国按蚊群（双翅目：蚊科）：核糖体 DNA 内转录间隔区 2 序列推断的物种区分和系统发育关系
影响因子：
2.1
作者：
Afrane, YA;Lawson, BW;Yan, GY
通讯作者：
Yan, GY