Shikonin Inhibits Fin Regeneration in Zebrafish Larvae.
Shikonin Inhibits Fin Regeneration in Zebrafish Larvae.
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紫草素抑制斑马鱼幼虫的鳍再生
DOI：
10.3390/cells11203187
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发表时间：
2022-10-11
期刊：
影响因子：
6
通讯作者：
Lu, Huiqiang
中科院分区：
文献类型：
--
作者：
Cao, Zigang;Guo, Chen;Chen, Guilan;Liu, Jiejun;Ni, Huiwen;Liu, Fangsheng;Xiong, Guanghua;Liao, Xinjun;Lu, Huiqiang
Shikonin is a naphthoquinone compound extracted from Chinese comfrey for treating cancer. However, there are few reports on its research on vertebrate tissue regeneration. Zebrafish is an ideal model for studying organ regeneration. In this study, we found that 3-dpf of zebrafish larvae exposed to shikonin at concentrations of 0.2, 0.3, and 0.4 mg/L showed increasingly inhibited regeneration of the tail fin. Immunohistochemical staining showed that shikonin exposure from 6 to 12 hpa increased the number of apoptotic cells in the caudal fin wound of larvae and decreased the number of proliferating cells. Shikonin exposure was found to up-regulate oxidative stress, increase ROS levels, and reduce neutrophil recruitment in the early stage of wound repair. Moreover, shikonin exposure caused disordered expression of fin regeneration blastemal-related genes. The use of astaxanthin to down-regulate oxidative stress was found to significantly reduce the inhibition of caudal fin regeneration. Mixed exposure of AMPK inhibitors or fullerenes (C60) with shikonin also showed the similar rescue effect. Collectively, our study showed that shikonin inhibited fin regeneration in zebrafish larvae by the upregulation of oxidative stress level and AMPK signaling pathway. This research provides valuable information on the mechanism of action of shikonin for its safe application.
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DOI：
10.1002/reg2.33
发表时间：
2015-04
期刊：
Regeneration (Oxford, England)
影响因子：
--
作者：
Pfefferli C;Jaźwińska A
通讯作者：
Jaźwińska A
影响因子：
4.5
作者：
Park JS;Chyun JH;Kim YK;Line LL;Chew BP
通讯作者：
Chew BP
影响因子：
2.5
作者：
Kawakami, A;Fukazawa, T;Takeda, H
通讯作者：
Takeda, H
影响因子：
2.6
作者：
Lan W;Wan S;Gu W;Wang H;Zhou S
通讯作者：
Zhou S
影响因子：
64.8
作者：
Green, Douglas R.;Kroemer, Guido
通讯作者：
Kroemer, Guido