Magnesium Oxide Nanoparticles: Effective Agricultural Antibacterial Agent Against Ralstonia solanacearum.
Magnesium Oxide Nanoparticles: Effective Agricultural Antibacterial Agent Against Ralstonia solanacearum.
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DOI：
10.3389/fmicb.2018.00790
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发表时间：
2018
影响因子：
5.2
通讯作者：
Ding W
中科院分区：
文献类型：
--
作者：
Cai L;Chen J;Liu Z;Wang H;Yang H;Ding W
镁（Mg）是植物必需的矿物质元素，对生物体无毒。在本研究中，我们利用纳米技术，首次在体外和体内系统研究了氧化镁纳米颗粒（MgONPs）对植物病原菌青枯菌（R. solanacearum）的抗菌机制。青枯菌导致了灾难性的青枯病，导致世界范围内的烟草产量减少。结果表明，MgONPs具有统计学上显着的浓度依赖性抗菌活性，最低抑菌浓度（MIC）和最低杀菌浓度（MBC）分别为200和250 μg/mL。旨在了解 MgONPs 毒性机制的其他研究表明，由于 MgONPs 直接附着在细菌细胞表面，细胞膜发生物理损伤，同时青枯菌的运动性和生物膜形成能力下降，这是通过扫描电子显微镜 (SEM) 和透射电子显微镜 (TEM) 观察到的。活性氧（ROS）的积累也可能是抗菌作用的一个重要原因，会诱导 DNA 损伤。温室条件下的毒性评估试验表明，MgONPs对烟草青枯病有很大的作用，降低了青枯病指数。总而言之，这些结果表明，开发 MgONPs 作为替代抗菌剂将成为一个新的研究课题。
Magnesium (Mg) is an essential mineral element for plants and is nontoxic to organisms. In this study, we took advantage of nanotechnologies to systematically investigate the antibacterial mechanisms of magnesium oxide nanoparticles (MgONPs) against the phytopathogen Ralstonia solanacearum (R. solanacearum) in vitro and in vivo for the first time. R. solanacearum has contributed to catastrophic bacterial wilt, which has resulted in the world-wide reduction of tobacco production. The results demonstrated that MgONPs possessed statistically significant concentration-dependent antibacterial activity, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured as 200 and 250 μg/mL, respectively. Additional studies, aimed at understanding the toxicity mechanism of MgONPs, indicated that physical injury occurred to the cell membranes, along with decreased motility and biofilm formation ability of R. solanacearum, due to the direct attachment of MgONPs to the surfaces of the bacterial cells, which was observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Reactive oxygen species (ROS) accumulation could also be an important reason for the antibacterial action, inducing DNA damage. The toxicity assessment assay under greenhouse conditions demonstrated that the MgONPs had exerted a large effect on tobacco bacterial wilt, reducing the bacterial wilt index. Altogether, the results suggest that the development of MgONPs as alternative antibacterial agents will become a new research subject.
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