Prediction and Analysis of SARS-CoV-2-Targeting MicroRNA in Human Lung Epithelium.
Prediction and Analysis of SARS-CoV-2-Targeting MicroRNA in Human Lung Epithelium.
复制标题
DOI：
10.3390/genes11091002
复制
发表时间：
2020-08-26
期刊：
影响因子：
3.5
通讯作者：
Salmena L
中科院分区：
文献类型：
--
作者：
Chow JT;Salmena L
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）是一种RNA病毒，是2020年冠状病毒病2019（COVID-19）大流行的原因。实验证据表明，microRNA可以介导针对某些RNA病毒的细胞内防御机制。本研究的目的是鉴定SARS-CoV-2基因组中具有预测结合位点的microRNA，将其与肺上皮组织中的microRNA表达谱进行比较，并对microRNA在减轻冠状病毒感染中的可能作用进行推断。我们推测，肺上皮细胞中特异性冠状病毒靶向microRNA的高表达可能保护免受感染和病毒繁殖，相反，低表达可能赋予感染易感性。我们已经鉴定了128种有可能靶向SARS-CoV-2基因组的人类microRNA，其中大多数在肺上皮中表达非常低。这128种microRNA中有6种在体外感染SARS-CoV-2后差异表达。此外，28种microRNA也靶向SARS-CoV基因组，而23种microRNA靶向MERS-CoV基因组。我们还发现，在另外两项研究中通常会发现一些microRNA。鉴定真正的冠状病毒靶向microRNA的进一步研究将有助于理解microRNA作为对抗病原性冠状病毒感染的细胞防御机制的重要性。
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), an RNA virus, is responsible for the coronavirus disease 2019 (COVID-19) pandemic of 2020. Experimental evidence suggests that microRNA can mediate an intracellular defence mechanism against some RNA viruses. The purpose of this study was to identify microRNA with predicted binding sites in the SARS-CoV-2 genome, compare these to their microRNA expression profiles in lung epithelial tissue and make inference towards possible roles for microRNA in mitigating coronavirus infection. We hypothesize that high expression of specific coronavirus-targeting microRNA in lung epithelia may protect against infection and viral propagation, conversely, low expression may confer susceptibility to infection. We have identified 128 human microRNA with potential to target the SARS-CoV-2 genome, most of which have very low expression in lung epithelia. Six of these 128 microRNA are differentially expressed upon in vitro infection of SARS-CoV-2. Additionally, 28 microRNA also target the SARS-CoV genome while 23 microRNA target the MERS-CoV genome. We also found that a number of microRNA are commonly identified in two other studies. Further research into identifying bona fide coronavirus targeting microRNA will be useful in understanding the importance of microRNA as a cellular defence mechanism against pathogenic coronavirus infections.
登录
查看更多内容
影响因子：
30.3
作者：
Morales L;Oliveros JC;Fernandez-Delgado R;tenOever BR;Enjuanes L;Sola I
通讯作者：
Sola I
DOI：
10.1038/nrmicro.2016.81
发表时间：
2016-08
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
de Wit E;van Doremalen N;Falzarano D;Munster VJ
通讯作者：
Munster VJ
影响因子：
5.1
作者：
Bhatti, Jasvinder Singh;Bhatti, Gurjit Kaur;Reddy, P. Hemachandra
通讯作者：
Reddy, P. Hemachandra
影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
Cao, Bin
影响因子：
16.8
作者：
Long, Dang;Lee, Rosalind;Ding, Ye
通讯作者：
Ding, Ye