The two-component system TarR-TarS is regulated by c-di-GMP/FleQ and FliA and modulates antibiotic susceptibility in Pseudomonas putida.
The two-component system TarR-TarS is regulated by c-di-GMP/FleQ and FliA and modulates antibiotic susceptibility in Pseudomonas putida.
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双组分系统 TarR-TarS 受 c-di-GMP/FleQ 和 FliA 调节，并调节恶臭假单胞菌的抗生素敏感性。
DOI：
10.1111/1462-2920.15555
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发表时间：
2021-05
影响因子：
5.1
通讯作者：
Qiaoyun Huang
中科院分区：
文献类型：
--
作者：
Yujie Xiao;Liang Nie;Haozhe Chen;Meina He;Qingyuan Liang;Hailing Nie;Wenli Chen;Qiaoyun Huang
双组分系统(TCS)是细菌感知和响应环境信号的主要方式。恶臭假单胞菌基因组包含数十个编码TCS的基因，但这些基因的表型-基因相关性和转录调控在很大程度上是未知的。在此，我们对一个保守的恶臭假单胞菌TCS的功能和转录调控进行了研究，命名为Tarr-Tars。TARS(PP_0769)编码一个潜在的组氨酸激酶，而TARR(PP_0768)编码一个潜在的反应调节因子。蛋白质相互作用实验和磷酸化实验证实TARR-TARS是一种功能性TCS。在抗生素作用下的生长实验表明，Tarr-Tars对细菌对多种抗生素的耐药性有正向调节作用。下拉实验表明，TARR与假想蛋白PP_0800和伴侣蛋白GroEL直接相互作用。GroEL对抗生素耐药有积极作用，而PP_0800对抗生素耐药性无明显影响。调节因子FleQ间接激活Tarr-Tars转录。然而，第二信使c-di-GMP拮抗FleQ的激活，从而抑制Tarr-tars的转录。Sigma因子FliA通过一个一致的基序直接激活Tarr-Tars转录。这些发现揭示了Tarr-tars的功能和转录调控，丰富了恶臭螺旋体c-di-GMP与抗生素耐药性关系的知识。
Two-component systems (TCSs) are predominant means by which bacteria sense and respond to environment signals. Genome of Pseudomonas putida contains dozens of putative TCS-encoding genes, but phenotypical-genotypical correlation and transcriptional regulation of these genes are largely unknown. Herein, we characterized function and transcriptional regulation of a conserved P. putida TCS, named TarR-TarS. TarS (PP_0769) encodes a potential histidine kinase, and tarR (PP_0768) encodes a potential response regulator. Protein-protein interaction assay and phosphorylation assay confirmed that TarR-TarS was a functional TCS. Growth assay under antibiotics revealed that TarR-TarS positively regulated bacterial resistance to multiple antibiotics. Pull-down assay revealed that TarR directly interacted with PP_0800 (a hypothetical protein) and GroEL (the chaperonin). GroEL played a positive role in antibiotic resistance, while PP_0800 seemed to have no effect on antibiotic resistance. The regulator FleQ indirectly activated tarR-tarS transcription. However, the second messenger c-di-GMP antagonized FleQ activation to inhibit tarR-tarS transcription. The sigma factor FliA directly activated tarR-tarS transcription via a consensus motif. These findings reveal function and transcriptional regulation of TarR-TarS, and enrich knowledge regarding the relationship between c-di-GMP and antibiotic resistance in P. putida.
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影响因子：
1.6
作者：
Hardjo Lugito NP;Nawangsih C;Moksidy JC;Kurniawan A;Tjiang MM
通讯作者：
Tjiang MM
影响因子：
3.2
作者：
Dasgupta, N;Ramphal, R
通讯作者：
Ramphal, R
影响因子：
3.2
作者：
J. Y. Park;S. Han;J. Lee;Y. Han;Yong Seok Lee;Xiaoqing Rong;Brian B. McSpadden Gardener;Hong-Seog Park;Young Cheol Kim
通讯作者：
J. Y. Park;S. Han;J. Lee;Y. Han;Yong Seok Lee;Xiaoqing Rong;Brian B. McSpadden Gardener;Hong-Seog Park;Young Cheol Kim
影响因子：
3.3
作者：
J. Rodríguez-Herva;E. Duque;M. A. Molina-Henares;Gloria Navarro-Avilés;P. Dillewijn;J. Torre;A. Molina-Henares;A. S. D. L. Campa;A. S. D. L. Campa;F. Ran;A. Segura;V. Shingler;J. Ramos;J. Ramos
通讯作者：
J. Rodríguez-Herva;E. Duque;M. A. Molina-Henares;Gloria Navarro-Avilés;P. Dillewijn;J. Torre;A. Molina-Henares;A. S. D. L. Campa;A. S. D. L. Campa;F. Ran;A. Segura;V. Shingler;J. Ramos;J. Ramos
影响因子：
5.2
作者：
Chuanqing Zhong;Peipei Zhang;Cheng Liu;Meng Liu;Wen-bing Chen;Jiafang Fu;Xiaoyu Qi;Guangxiang Cao-Guang
通讯作者：
Chuanqing Zhong;Peipei Zhang;Cheng Liu;Meng Liu;Wen-bing Chen;Jiafang Fu;Xiaoyu Qi;Guangxiang Cao-Guang