Activity-dependent gating of calcium spikes by A-type K+ channels controls climbing fiber signaling in Purkinje cell dendrites.
Activity-dependent gating of calcium spikes by A-type K+ channels controls climbing fiber signaling in Purkinje cell dendrites.
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DOI：
10.1016/j.neuron.2014.08.035
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发表时间：
2014-10-01
期刊：
影响因子：
16.2
通讯作者：
Dieudonne, Stephane
中科院分区：
文献类型：
--
作者：
Otsu, Yo;Marcaggi, Paikan;Feltz, Anne;Isope, Philippe;Kollo, Mihaly;Nusser, Zoltan;Mathieu, Benjamin;Kano, Masanobu;Tsujita, Mika;Sakimura, Kenji;Dieudonne, Stephane
在小脑浦肯野细胞树突中，近端攀爬纤维（CF）输入诱导的异突触钙信号控制远端平行纤维（PF）突触的可塑性。这种长距离树突状细胞钙信号的底物和调节机制还知之甚少。使用高速钙成像，我们研究的作用，积极的树突状电导。在基础条件下，CF刺激诱发T型钙信号传导，显示急剧的近远侧递减。结合mGluR 1受体激活和去极化，两种活性依赖性信号，解锁P/Q钙峰的起始和传播，介导远端部位的有效CF信号传导。这些尖峰起始于近端光滑树突，独立于体细胞钠动作电位，并引起PF棘中所有或无快速上升钙瞬变的高频爆发。逐渐的钙峰爆发解锁来自于位于远端树突的mGluR 1调节的低阈值A型钾通道的失活增加。PF突触的分级活动依赖性CF钙信号传导的证据完善了目前对小脑监督学习规则的看法。巨大的近端CF输入控制远端PF-浦肯野突触的Ca 2+可塑性我们发现mGluR 1活性和浦肯野细胞去极化控制CF钙内流远端CF信号是由离心树突钙峰爆发介导的树突低阈值A型K+通道调节是钙峰门控的基础爬肌纤维监督小脑学习，在浦肯野细胞上的平行突触表现为钙依赖性可塑性。大津等人发现活动依赖性参数通过门控浦肯野细胞树突中的钙峰来控制异突触攀爬纤维信号传导。
In cerebellar Purkinje cell dendrites, heterosynaptic calcium signaling induced by the proximal climbing fiber (CF) input controls plasticity at distal parallel fiber (PF) synapses. The substrate and regulation of this long-range dendritic calcium signaling are poorly understood. Using high-speed calcium imaging, we examine the role of active dendritic conductances. Under basal conditions, CF stimulation evokes T-type calcium signaling displaying sharp proximodistal decrement. Combined mGluR1 receptor activation and depolarization, two activity-dependent signals, unlock P/Q calcium spikes initiation and propagation, mediating efficient CF signaling at distal sites. These spikes are initiated in proximal smooth dendrites, independently from somatic sodium action potentials, and evoke high-frequency bursts of all-or-none fast-rising calcium transients in PF spines. Gradual calcium spike burst unlocking arises from increasing inactivation of mGluR1-modulated low-threshold A-type potassium channels located in distal dendrites. Evidence for graded activity-dependent CF calcium signaling at PF synapses refines current views on cerebellar supervised learning rules. The giant proximal CF input controls Ca2+ plasticity at distal PF-Purkinje synapses We find mGluR1 activity and Purkinje cell depolarization to control CF calcium influx Distal CF signaling is mediated by centrifugal dendritic calcium spikes bursts Dendritic low-threshold A-type K+ channels modulation underlie calcium spike gating Climbing fibers supervise cerebellar learning expressed as calcium-dependent plasticity at the parallel synapses on Purkinje cells. Otsu et al. find that activity-dependent parameters control heterosynaptic climbing fiber signaling by gating calcium spikes in Purkinje cell dendrites.
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神经元表面A型K+通道的新型亚细胞分布模式。
影响因子：
5.3
作者：
Kollo, Mihaly;Holderith, Noemi B;Nusser, Zoltan
通讯作者：
Nusser, Zoltan
影响因子：
2.5
作者：
Bengtsson, Fredrik;Joerntell, Henrik
通讯作者：
Joerntell, Henrik
影响因子：
5.3
作者：
Anwar, Haroon;Hepburn, Iain;De Schutter, Erik
通讯作者：
De Schutter, Erik
影响因子：
64.8
作者：
Finch, EA;Augustine, GJ
通讯作者：
Augustine, GJ
DOI：
10.1073/pnas.0808428106
发表时间：
2009-02-17
影响因子：
11.1
作者：
Bengtsson, Fredrik;Jorntell, Henrik
通讯作者：
Jorntell, Henrik