In Vitro Human Haematopoietic Stem Cell Expansion and Differentiation.
In Vitro Human Haematopoietic Stem Cell Expansion and Differentiation.
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造血系统在我们的健康和生存中起着至关重要的作用。它由一系列成熟的血液和免疫细胞类型组成，包括携氧红细胞、产生血小板的巨核细胞和抗感染的骨髓和淋巴细胞。自我更新的多能造血干细胞（HSC）和一系列中间造血祖细胞类型分化成这些成熟细胞类型，以在整个生命中持续支持造血系统稳态。造血过程在体内受到严格调控，主要发生在骨髓中。多年来，基于重组细胞因子、共培养系统和/或小分子的使用，已经开发了一系列体外培养系统，以扩增造血干细胞和祖细胞或将它们分化成各种造血谱系。这些方法提供了重要的易处理的模型来研究体外人类造血。此外，造血细胞培养系统正在开发和临床试验，作为移植和输血医学的细胞产品来源。本文综述了体外培养人HSC扩增和分化的方法，并总结了参与这些生物学过程的关键因素。
The haematopoietic system plays an essential role in our health and survival. It is comprised of a range of mature blood and immune cell types, including oxygen-carrying erythrocytes, platelet-producing megakaryocytes and infection-fighting myeloid and lymphoid cells. Self-renewing multipotent haematopoietic stem cells (HSCs) and a range of intermediate haematopoietic progenitor cell types differentiate into these mature cell types to continuously support haematopoietic system homeostasis throughout life. This process of haematopoiesis is tightly regulated in vivo and primarily takes place in the bone marrow. Over the years, a range of in vitro culture systems have been developed, either to expand haematopoietic stem and progenitor cells or to differentiate them into the various haematopoietic lineages, based on the use of recombinant cytokines, co-culture systems and/or small molecules. These approaches provide important tractable models to study human haematopoiesis in vitro. Additionally, haematopoietic cell culture systems are being developed and clinical tested as a source of cell products for transplantation and transfusion medicine. This review discusses the in vitro culture protocols for human HSC expansion and differentiation, and summarises the key factors involved in these biological processes.
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影响因子：
4.3
作者：
de Souza VCA;Pereira TA;Teixeira VW;Carvalho H;de Castro MCAB;D'assunção CG;de Barros AF;Carvalho CL;de Lorena VMB;Costa VMA;Teixeira ÁAC;Figueiredo RCBQ;de Oliveira SA
通讯作者：
de Oliveira SA
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30.8
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6.5
作者：
Arcasoy, MO;Jiang, XH
通讯作者：
Jiang, XH
影响因子：
3.1
作者：
Almendras, Carmen P. Montano;Thudium, Christian S.;Richter, Johan
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15.3
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