Development and characterization of 18 novel EST-SSRs from the western flower Thrips, Frankliniella occidentalis (Pergande).
Development and characterization of 18 novel EST-SSRs from the western flower Thrips, Frankliniella occidentalis (Pergande).
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DOI：
10.3390/ijms13032863
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发表时间：
2012
影响因子：
5.6
通讯作者：
Hong XY
中科院分区：
文献类型：
--
作者：
Yang XM;Sun JT;Xue XF;Zhu WC;Hong XY
西部花蓟马（Frankliniella occidentalis, Pergande）是一种外来入侵物种，是蓟马目昆虫中最重要的经济害虫。为了更好地了解西方F. occidentalis在世界范围内的遗传组成和迁移模式，我们对18个新的est衍生的多态性微卫星进行了表征。研究了这些EST-SSRs的突变机制，为群体遗传研究选择合适的标记组合提供了便利。在中国哈尔滨、大理和贵阳三个种群的96个个体中对这些新标记进行了遗传多样性评估。结果表明，这18个基因座均具有高度多态性；等位基因数量在2 ~ 15个之间，平均每个位点有5.50个等位基因。观察杂合度（HO）为0.072 ~ 0.707，期望杂合度（HE）为0.089 ~ 0.851。只有大理的WFT144和贵阳的WFT50显著偏离Hardy-Weinberg平衡（HWE）。两两FST分析显示，在所有三个两两群体比较中，差异低但显著（0.026 < FST < 0.032）。每个位点等位基因的序列分析显示，这些EST-SSRs具有复杂的突变模式。因此，这些EST-SSRs是有用的标记，但在用于群体遗传研究时，应更加注意这些微卫星的突变特征。
The western flower thrips, Frankliniella occidentalis (Pergande), is an invasive species and the most economically important pest within the insect order Thysanoptera. For a better understanding of the genetic makeup and migration patterns of F. occidentalis throughout the world, we characterized 18 novel polymorphic EST-derived microsatellites. The mutational mechanism of these EST-SSRs was also investigated to facilitate the selection of appropriate combinations of markers for population genetic studies. Genetic diversity of these novel markers was assessed in 96 individuals from three populations in China (Harbin, Dali, and Guiyang). The results showed that all these 18 loci were highly polymorphic; the number of alleles ranged from 2 to 15, with an average of 5.50 alleles per locus. The observed (HO) and expected (HE) heterozygosities ranged from 0.072 to 0.707 and 0.089 to 0.851, respectively. Furthermore, only two locus/population combinations (WFT144 in Dali and WFT50 in Guiyang) significantly deviated from Hardy–Weinberg equilibrium (HWE). Pairwise FST analysis showed a low but significant differentiation (0.026 < FST < 0.032) among all three pairwise population comparisons. Sequence analysis of alleles per locus revealed a complex mutational pattern of these EST-SSRs. Thus, these EST-SSRs are useful markers but greater attention should be paid to the mutational characteristics of these microsatellites when they are used in population genetic studies.
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