Thin-walled microvessels in human coronary atherosclerotic plaques show incomplete endothelial junctions relevance of compromised structural integrity for intraplaque microvascular leakage.
Thin-walled microvessels in human coronary atherosclerotic plaques show incomplete endothelial junctions relevance of compromised structural integrity for intraplaque microvascular leakage.
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DOI：
10.1016/j.jacc.2008.12.056
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发表时间：
2009-04-28
影响因子：
24
通讯作者：
Daemen, Mat J. A. P.
中科院分区：
文献类型：
--
作者：
Sluimer, Judith C.;Kolodgie, Frank D.;Bijnens, Ann P. J. J.;Maxfield, Kimberly;Pacheco, Erica;Kutys, Bob;Duimel, Hans;Frederik, Peter M.;van Hinsbergh, Victor W. M.;Virmani, Renu;Daemen, Mat J. A. P.
探讨正常和粥样硬化冠状动脉微血管的超微结构及其与斑块表型的关系。动脉粥样硬化斑块中的微血管是炎性细胞和红细胞的进入点。然而，关于人类动脉粥样硬化中微血管超微结构完整性的数据有限。采用免疫组化法测定28支冠状动脉的外膜、内膜-中膜边界和动脉粥样硬化斑块中的微血管密度（MVD）和超微结构形态，包括内皮细胞（荆豆，CD 31/CD 34）、基底膜（层粘连蛋白，胶原IV）和壁细胞（结蛋白，α-平滑肌（SM）肌动蛋白，平滑肌蛋白，SM 1，SM 2，Smmember）。用电镜观察微血管的超微结构。与早期斑块相比，晚期斑块中MVD增加，这与病变形态学相关。正常动脉和早期斑块外膜MVD高于斑块内MVD，但晚期斑块外膜和斑块内MVD相似。虽然微血管基底膜是完整的，薄壁微血管的百分比同样低，在正常和动脉粥样硬化外膜，在外膜和斑块，并在所有斑块类型。斑块内微血管内皮细胞（EC）异常，有膜泡、胞浆内空泡、开放的EC-EC连接和基底膜脱离。电镜下可见大量白细胞浸润，免疫组化CD 45 RO和CD 68染色证实。MVD与冠状动脉斑块进展和形态学相关。正常和动脉粥样硬化动脉中的微血管是薄壁的，微血管内皮的结构完整性受损可以解释晚期人类冠状动脉粥样硬化斑块内出血的微血管渗漏。
To examine the ultrastructure of microvessels in normal and atherosclerotic coronary arteries and its association with plaque phenotype. Microvessels in atherosclerotic plaques are an entry point for inflammatory and red blood cells. Yet there is limited data on the ultrastructural integrity of microvessels in human atherosclerosis. Microvessel density (MVD) and ultrastructural morphology were determined in the adventitia, intima-media border, and atherosclerotic plaque of 28 coronary arteries using immunohistochemistry for endothelial cells (Ulex europeaus, CD31/CD34), basement membrane (laminin, collagen IV), and mural cells (desmin, alpha-smooth muscle (SM) actin, smoothelin, SM1, SM2, Smemb). Ultrastructural characterization of microvessel morphology was performed by electron microscopy (EM). MVD was increased in advanced plaques compared to early plaques, which correlated with lesion morphology. Adventitial MVD was higher than intraplaque MVD in normal arteries and early plaques, but adventitial and intraplaque MVD were similar in advanced plaques. Although microvessel basement membranes were intact, the percentage of thin-walled microvessels was similarly low in normal and atherosclerotic adventitia, in the adventitia and the plaque, and in all plaque types. Intraplaque microvascular endothelial cells (EC) were abnormal, with membrane blebs, intracytoplasmic vacuoles, open EC-EC junctions, and basement membrane detachment. Leukocyte infiltration was frequently observed by EM, and confirmed by CD45RO and CD68 immunohistochemistry. MVD was associated with coronary plaque progression and morphology. Microvessels were thin-walled in normal and atherosclerotic arteries, and the compromised structural integrity of microvascular endothelium may explain the microvascular leakage responsible for intraplaque hemorrhage in advanced human coronary atherosclerosis.
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DOI：
10.1161/01.atv.0000140820.51174.8d
发表时间：
2004-10-01
影响因子：
8.7
作者：
Lappalainen, H;Laine, P;Kovanen, PT
通讯作者：
Kovanen, PT
影响因子：
37.8
作者：
Khurana, R;Moons, L;Zachary, IC
通讯作者：
Zachary, IC
影响因子：
3.5
作者：
Mazzone, Annamaria;Epistolato, Maria Carmela;Tanganelli, Piero
通讯作者：
Tanganelli, Piero
缺乏周细胞会导致内皮增生和异常血管形态发生。
影响因子：
7.8
作者：
Hellstrom, M;Gerhardt, H;Kalen, M;Li, X;Eriksson, U;Wolburg, H;Betsholtz, C
通讯作者：
Betsholtz, C
DOI：
10.1161/01.atv.0000197795.56960.64
发表时间：
2006-02-01
影响因子：
8.7
作者：
de Nooijer, R;Verkleij, CJN;Biessen, EAL
通讯作者：
Biessen, EAL