Comparative Analysis of Structural Features in SLiMs from Eukaryotes, Bacteria, and Viruses with Importance for Host-Pathogen Interactions.
Comparative Analysis of Structural Features in SLiMs from Eukaryotes, Bacteria, and Viruses with Importance for Host-Pathogen Interactions.
复制标题
来自真核生物、细菌和病毒的SLiM结构特征的比较分析，对宿主-病原体相互作用具有重要意义。
DOI：
10.3390/pathogens11050583
复制
发表时间：
2022-05-15
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
蛋白质-蛋白质相互作用驱动真核生物中的功能，这些功能可以通过短线性基序（SLiM）来描述。SLiM的保守性有助于阐明真核蛋白家族中的功能性SLiM。然而，真核SLiM的简单性使得它们偶然出现，这是由于不仅在真核生物中而且在致病细菌和病毒中的突变过程。此外，功能性真核SLiM通常在无序区域中发现。虽然病原细菌和病毒的蛋白质组比真核蛋白质组具有更少的紊乱，但它们的蛋白质可以成功地模拟真核SLiM并破坏宿主细胞功能。识别病原体中重要的SLiM是困难的，但对于理解潜在的宿主-病原体相互作用是必不可少的。我们对来自真核线性基序（ELM）数据库的实验验证的SLiM在病毒、细菌和真核生物中的结构特征进行了比较分析。我们的研究结果表明，许多病毒SLiM和跨病毒和真核生物发现的特定基序，如一些糖基化基序，具有较少的混乱。分析病原体和真核生物的等效SLiM的无序和卷曲特性发现，病原体中的一些基序比真核生物中的基序结构更复杂，反之亦然。这些结果支持病原体和它们的真核宿主之间的相互作用的不同机制的一些相同的图案。
Protein-protein interactions drive functions in eukaryotes that can be described by short linear motifs (SLiMs). Conservation of SLiMs help illuminate functional SLiMs in eukaryotic protein families. However, the simplicity of eukaryotic SLiMs makes them appear by chance due to mutational processes not only in eukaryotes but also in pathogenic bacteria and viruses. Further, functional eukaryotic SLiMs are often found in disordered regions. Although proteomes from pathogenic bacteria and viruses have less disorder than eukaryotic proteomes, their proteins can successfully mimic eukaryotic SLiMs and disrupt host cellular function. Identifying important SLiMs in pathogens is difficult but essential for understanding potential host-pathogen interactions. We performed a comparative analysis of structural features for experimentally verified SLiMs from the Eukaryotic Linear Motif (ELM) database across viruses, bacteria, and eukaryotes. Our results revealed that many viral SLiMs and specific motifs found across viruses and eukaryotes, such as some glycosylation motifs, have less disorder. Analyzing the disorder and coil properties of equivalent SLiMs from pathogens and eukaryotes revealed that some motifs are more structured in pathogens than their eukaryotic counterparts and vice versa. These results support a varying mechanism of interaction between pathogens and their eukaryotic hosts for some of the same motifs.
登录
查看更多内容
影响因子：
5.6
作者：
Chemes, Lucia B.;Sanchez, Ignacio E.;de Prat-Gay, Gonzalo
通讯作者：
de Prat-Gay, Gonzalo
DOI：
10.1186/s12964-015-0120-z
发表时间：
2015-11-21
期刊：
Cell communication and signaling : CCS
影响因子：
--
作者：
Davey NE;Cyert MS;Moses AM
通讯作者：
Moses AM
DOI：
10.3390/v13122369
发表时间：
2021-11-26
期刊：
Viruses
影响因子：
--
作者：
Elkhaligy H;Balbin CA;Gonzalez JL;Liberatore T;Siltberg-Liberles J
通讯作者：
Siltberg-Liberles J
影响因子：
14.9
作者：
Gould CM;Diella F;Via A;Puntervoll P;Gemünd C;Chabanis-Davidson S;Michael S;Sayadi A;Bryne JC;Chica C;Seiler M;Davey NE;Haslam N;Weatheritt RJ;Budd A;Hughes T;Pas J;Rychlewski L;Travé G;Aasland R;Helmer-Citterich M;Linding R;Gibson TJ
通讯作者：
Gibson TJ
影响因子：
--
作者：
Davey, Norman E.;Van Roey, Kim;Gibson, Toby J.
通讯作者：
Gibson, Toby J.