Receptors for insulin-like growth factors and growth effects of multiplication-stimulating activity (rat insulin-like growth factor II) in rat embryo fibroblasts.
Receptors for insulin-like growth factors and growth effects of multiplication-stimulating activity (rat insulin-like growth factor II) in rat embryo fibroblasts.
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大鼠胚胎成纤维细胞中胰岛素样生长因子的受体和增殖刺激活性（大鼠胰岛素样生长因子 II）的生长效应。
DOI：
10.1210/endo-112-3-971
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发表时间：
1983
期刊：
影响因子：
4.8
通讯作者：
M. Rechler
中科院分区：
文献类型：
--
作者：
S. O. Adams;S. Nissley;M. Kasuga;T. Foley;M. Rechler
胎鼠血清中的增殖刺激活性(MSA)水平较高(2-4微克/毫升)，提示MSA可能在胎儿生长发育中起作用。我们现在证明了来自大鼠胚胎的成纤维细胞(ReFS)具有特异性的MSA受体，并通过增加DNA合成来响应MSA。通过放射性碘标记的MSA、IGF-I和IGF-II的竞争结合以及[125I]Ido-MSA和[125I]IGF-I与ReFs的化学交联，证实了两种类型的胰岛素样生长因子(IGF)受体。一种类型的受体(在还原条件下，摩尔重量为260,000)不与胰岛素相互作用，另一种类型的受体(在还原条件下，摩尔重量为130,000)可被胰岛素识别。[~(125)I]I-MSA结合数据的Scatchard分析符合一类非相互作用的结合位点。文献用放射自显影证实了MSA的生物学效应，即DNA合成增加。在16小时孵育过程中，正常大鼠血清、低血小板血浆加血小板衍生生长因子(PDGF)刺激DNA合成，但不受去垂体大鼠血清或去垂体大鼠低血小板血浆加PDGF刺激。然而，当MSA加入低氧血清或低氧低血小板血浆加PDGF时，DNA合成被刺激到正常大鼠血清的水平。相比之下，在较长时间的细胞增殖实验中，REFS在正常或低氧大鼠血清中生长同样良好，这增加了REFS可能产生MSA样因子的可能性。
Levels of multiplication-stimulating activity (MSA) in fetal rat serum are high (2-4 micrograms/ml), suggesting that MSA may have a role in fetal growth. We now demonstrate that fibroblasts derived from rat embryos (REFs) have specific MSA receptors and respond to MSA with increased DNA synthesis. Two types of insulin-like growth factor (IGF) receptors were demonstrated by competitive binding of radioiodinated MSA, IGF-I, and IGF-II and by chemical cross-linking of [125I]iodo-MSA and [125I]iodo-IGF-I to REFs. One type of receptor (mol wt, 260,000 under reducing conditions) did not interact with insulin, and another type of receptor (mol wt, 130,000, under reducing conditions) was recognized by insulin. Scatchard analysis of [125I]iodo-MSA binding data was consistent with one class of noninteracting binding sites. A biological response of MSA, increased DNA synthesis, was demonstrated with autoradiography in REFs. During a 16-hr incubation, DNA synthesis was stimulated by normal rat serum, and platelet-poor plasma plus platelet-derived growth factor (PDGF), but not by serum from hypophysectomized (hypox) rats or hypophysectomized (hypox) platelet-poor plasma plus PDGF. However, when MSA was added to hypox serum or to hypox platelet-poor plasma plus PDGF, DNA synthesis was stimulated to the level achieved by normal rat serum. By contrast, during a longer cell multiplication experiment, REFs grew equally well in normal or hypox rat serum, raising the possibility that REFs may produce a MSA-like factor.
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DOI：
10.1073/pnas.76.4.1809
发表时间：
1979-01-01
影响因子：
11.1
作者：
ANTONIADES, HN;SCHER, CD;STILES, CD
通讯作者：
STILES, CD
大鼠和人体组织膜中增殖刺激活性受体的亲和标记。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Massague,J;Guillette,BJ;Czech,MP
通讯作者：
Czech,MP
培养的人成纤维细胞产生免疫反应性生长调节素的激素控制。
DOI：
10.1172/jci110001
发表时间：
1981
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Clemmons,DR;Underwood,LE;VanWyk,JJ
通讯作者：
VanWyk,JJ
大鼠碱性生长调节素的分离和部分序列分析。
影响因子：
4.8
作者：
Rubin,JS;Mariz,I;Jacobs,JW;Daughaday,WH;Bradshaw,RA
通讯作者：
Bradshaw,RA