Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells.
Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells.
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DOI：
10.4103/1673-5374.145384
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发表时间：
2014-11-15
影响因子：
6.1
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Li Q;Lu J;Wang X
异丙酚和瑞芬太尼通过激活γ-氨基丁酸A型受体和降低睾酮水平改变神经干/祖细胞内Ca2+浓度（[Ca2+]i）。然而，这一过程是否影响神经干/祖细胞的增殖和分化仍然未知。在本研究中，我们将异丙酚和瑞芬太尼单独或联合应用低、中、高浓度（临床有效血药浓度的1、2-2.5和4-5倍）作用于新生大鼠幼崽海马的神经干/祖细胞。低浓度异丙酚、瑞芬太尼或两者均对细胞增殖和分化无明显影响；然而，中等和高浓度的异丙酚和/或瑞芬太尼显著抑制神经干/祖细胞的增殖和分化，并诱导神经干/祖细胞分化初期[Ca2+]i的减少。因此，我们提出异丙酚和瑞芬太尼通过改变[Ca2+]i来干扰神经干细胞/祖细胞的增殖和分化。我们的研究结果表明，在小儿麻醉中应谨慎使用异丙酚和/或瑞芬太尼。
Propofol and remifentanil alter intracellular Ca2+ concentration ([Ca2+]i) in neural stem/progenitor cells by activating γ-aminobutyric acid type A receptors and by reducing testosterone levels. However, whether this process affects neural stem/progenitor cell proliferation and differentiation remains unknown. In the present study, we applied propofol and remifentanil, alone or in combination, at low, moderate or high concentrations (1, 2–2.5 and 4–5 times the clinically effective blood drug concentration), to neural stem/progenitor cells from the hippocampi of newborn rat pups. Low concentrations of propofol, remifentanil or both had no noticeable effect on cell proliferation or differentiation; however, moderate and high concentrations of propofol and/or remifentanil markedly suppressed neural stem/progenitor cell proliferation and differentiation, and induced a decrease in [Ca2+]i during the initial stage of neural stem/progenitor cell differentiation. We therefore propose that propofol and remifentanil interfere with the proliferation and differentiation of neural stem/progenitor cells by altering [Ca2+]i. Our findings suggest that propofol and/or remifentanil should be used with caution in pediatric anesthesia.
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影响因子：
6.1
作者：
Lu J;Li D;Lu K
通讯作者：
Lu K
影响因子：
1.7
作者：
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影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
3.2
作者：
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通讯作者：
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