Analysis of complete genome sequence of Neorickettsia risticii: causative agent of Potomac horse fever.
Analysis of complete genome sequence of Neorickettsia risticii: causative agent of Potomac horse fever.
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DOI：
10.1093/nar/gkp642
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发表时间：
2009-10
影响因子：
14.9
通讯作者：
Rikihisa Y
中科院分区：
文献类型：
--
作者：
Lin M;Zhang C;Gibson K;Rikihisa Y
里氏奈氏立克次体是吸虫和哺乳动物的专性胞内细菌。当马摄取含有包囊的新城疫吸虫的水生昆虫时，马就会患上波托马克马瘟(PHF)。伊利诺伊州新城疫原虫的基因组全序列由一条879 977bp的单环染色体组成，编码38种RNA和898种蛋白质。虽然它合成氨基酸的能力有限，缺乏许多代谢途径，但它能够制造主要的维生素、辅因子和核苷酸。与其近缘人类病原菌的比较表明，758个蛋白质编码基因(88.2%)在里氏新城疫病菌和森氏新城疫病菌之间是保守的。四方比对表明，大多数基因要么在无浆科之间共享(525个同源基因，通常与管家功能相关)，要么专属于每个基因组(>；200个基因，大多是假设的蛋白质)。鉴定了可能与致病相关的基因，包括编码外膜蛋白、双组分系统和IV型分泌系统(T4SS)的基因。首次在专性胞内细菌中证实了T4SS菌毛蛋白VirB2在细菌表面的双极定位。这些数据为了解立氏奈瑟氏菌的基因组潜力和细胞内寄生提供了线索，并有助于我们理解PHF的发病机制。
Neorickettsia risticii is an obligate intracellular bacterium of the trematodes and mammals. Horses develop Potomac horse fever (PHF) when they ingest aquatic insects containing encysted N. risticii-infected trematodes. The complete genome sequence of N. risticii Illinois consists of a single circular chromosome of 879 977 bp and encodes 38 RNA species and 898 proteins. Although N. risticii has limited ability to synthesize amino acids and lacks many metabolic pathways, it is capable of making major vitamins, cofactors and nucleotides. Comparison with its closely related human pathogen N. sennetsu showed that 758 (88.2%) of protein-coding genes are conserved between N. risticii and N. sennetsu. Four-way comparison of genes among N. risticii and other Anaplasmataceae showed that most genes are either shared among Anaplasmataceae (525 orthologs that generally associated with housekeeping functions), or specific to each genome (>200 genes that are mostly hypothetical proteins). Genes potentially involved in the pathogenesis of N. risticii were identified, including those encoding putative outer membrane proteins, two-component systems and a type IV secretion system (T4SS). The bipolar localization of T4SS pilus protein VirB2 on the bacterial surface was demonstrated for the first time in obligate intracellular bacteria. These data provide insights toward genomic potential of N. risticii and intracellular parasitism, and facilitate our understanding of PHF pathogenesis.
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影响因子：
4.5
作者：
Dunning Hotopp JC;Lin M;Madupu R;Crabtree J;Angiuoli SV;Eisen JA;Seshadri R;Ren Q;Wu M;Utterback TR;Smith S;Lewis M;Khouri H;Zhang C;Niu H;Lin Q;Ohashi N;Zhi N;Nelson W;Brinkac LM;Dodson RJ;Rosovitz MJ;Sundaram J;Daugherty SC;Davidsen T;Durkin AS;Gwinn M;Haft DH;Selengut JD;Sullivan SA;Zafar N;Zhou L;Benahmed F;Forberger H;Halpin R;Mulligan S;Robinson J;White O;Rikihisa Y;Tettelin H
通讯作者：
Tettelin H
影响因子：
3.4
作者：
Cheng, Zhihui;Kumagai, Yumi;Rikihisa, Yasuko
通讯作者：
Rikihisa, Yasuko
影响因子：
14.9
作者：
Bagos, PG;Liakopoulos, TD;Hamodrakas, SJ
通讯作者：
Hamodrakas, SJ
影响因子：
3.1
作者：
Biswas, B;Vemulapalli, R;Dutta, SK
通讯作者：
Dutta, SK
影响因子：
4.5
作者：
Blanc, Guillaume;Ogata, Hiroyuki;Robert, Catherine;Audic, Stephane;Suhre, Karsten;Vestris, Guy;Claverie, Jean-Michel;Raoult, Didier
通讯作者：
Raoult, Didier