Similar antigen cross-presentation capacity and phagocytic functions in all freshly isolated human lymphoid organ-resident dendritic cells.
Similar antigen cross-presentation capacity and phagocytic functions in all freshly isolated human lymphoid organ-resident dendritic cells.
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DOI：
10.1084/jem.20121103
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发表时间：
2013-05-06
期刊：
影响因子：
--
通讯作者：
Amigorena S
中科院分区：
文献类型：
--
作者：
Segura E;Durand M;Amigorena S
扁桃体驻留的BDCA 1 + DC、BDCA 3 + DC和pDC都有效地交叉呈递抗原。树突状细胞（Dendritic cells，DC）是一种异质性抗原呈递细胞，在次级淋巴器官中启动和定向免疫应答。在小鼠中，淋巴器官驻留的CD8+ DC在交叉呈递方面特化，并且已经发展了其内吞途径的特异性适应（高pH、低降解和向胞质溶胶的高输出）。在人类中，血液BDCA 3 + DC最近被证明是小鼠CD8+ DC的同源物。还提出它们在体外活化后比其他血液DC亚群更有效地交叉提呈抗原，这表明在人类中交叉提呈仅限于某些DC亚群。然而，在生理上交叉呈递抗原的DC是存在于淋巴器官中的DC。在这里，我们表明，新鲜分离的扁桃体驻留BDCA 1 + DC，BDCA 3 + DC，和pDC都交叉有效地提出可溶性抗原相比，巨噬细胞，在没有激活。此外，BDCA 1+和BDCA 3 + DC在其吞噬体中显示出相似的吞噬体pH和相似的活性氧物质产生。与巨噬细胞相反，所有三个DC亚群也有效地将内化的蛋白质输出到胞质溶胶。我们的结论是，所有新鲜分离的淋巴器官居民的人树突状细胞，但不是巨噬细胞，显示高内在的交叉呈递能力。
Tonsil-resident BDCA1+ DCs, BDCA3+ DCs, and pDCs all cross-present antigen efficiently. Dendritic cells (DCs) represent a heterogeneous population of antigen-presenting cells that initiate and orient immune responses in secondary lymphoid organs. In mice, lymphoid organ–resident CD8+ DCs are specialized at cross-presentation and have developed specific adaptations of their endocytic pathway (high pH, low degradation, and high export to the cytosol). In humans, blood BDCA3+ DCs were recently shown to be the homologues of mouse CD8+ DCs. They were also proposed to cross-present antigens more efficiently than other blood DC subsets after in vitro activation, suggesting that in humans cross-presentation is restricted to certain DC subsets. The DCs that cross-present antigen physiologically, however, are the ones present in lymphoid organs. Here, we show that freshly isolated tonsil-resident BDCA1+ DCs, BDCA3+ DCs, and pDCs all cross-present soluble antigen efficiently, as compared to macrophages, in the absence of activation. In addition, BDCA1+ and BDCA3+ DCs display similar phagosomal pH and similar production of reactive oxygen species in their phagosomes. All three DC subsets, in contrast to macrophages, also efficiently export internalized proteins to the cytosol. We conclude that all freshly isolated lymphoid organ–resident human DCs, but not macrophages, display high intrinsic cross-presentation capacity.
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影响因子：
15.3
作者：
Bonifaz, L;Bonnyay, D;Mahnke, K;Rivera, M;Nussenzweig, MC;Steinman, RM
通讯作者：
Steinman, RM
DOI：
10.1084/jem.20081633
发表时间：
2009-02-16
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Haniffa M;Ginhoux F;Wang XN;Bigley V;Abel M;Dimmick I;Bullock S;Grisotto M;Booth T;Taub P;Hilkens C;Merad M;Collin M
通讯作者：
Collin M
DOI：
10.1073/pnas.0712394105
发表时间：
2008-02-26
影响因子：
11.1
作者：
Lin, Ming Lee;Zhan, Yifan;Lew, Andrew M.
通讯作者：
Lew, Andrew M.
影响因子：
15.9
作者：
Helft, Julie;Manicassamy, Balaji;Merad, Miriam
通讯作者：
Merad, Miriam
影响因子：
5.4
作者：
Backer, Ronald;van Leeuwen, Fred;den Haan, Joke M. M.
通讯作者：
den Haan, Joke M. M.