Pdh is involved in the cell division and Normal septation of Streptococcus suis.
Pdh is involved in the cell division and Normal septation of Streptococcus suis.
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Pdh 参与猪链球菌的细胞分裂和正常分隔。
DOI：
10.1016/j.micres.2019.126304
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发表时间：
2019-08
影响因子：
6.7
通讯作者：
Li Yi
中科院分区：
文献类型：
--
作者：
Wang Yang;Wang Yuxin;Li Jinpeng;Gong Shenglong;Sun Liyun;Grenier Daniel;Li Yi
猪链球菌（Streptococcus suis，S.猪流感是一种重要的人畜共患病原体，在世界范围内造成养猪业的重大经济损失。帖前细胞分裂过程是其生长和繁殖的重要组成部分，受一个复杂的调控网络控制。丙酮酸脱氢酶（PDH）催化丙酮酸氧化脱羧形成乙酰辅酶A，同时将NAD +还原为NADH，在能量代谢中起重要作用。最近，我们报道pdh通过降低猪链球菌血清2型的应激耐受性和生物膜形成来调节毒力。本研究发现，pdh基因缺失后，猪链球菌细胞链异常，细胞形态丰满，Z环定位异常，表明pdh基因缺失突变体的分裂能力受损。此外，通过ELISA方法证实了FtsZ与PDH在体外的相互作用，qRT-PCR分析表明pdh基因的缺失导致了分裂相关基因ftsZ、ftsK、ftsl、zapA、divIC、pbp 1a、rodA、mreD和sepF的差异表达。这些结果表明pdhi参与了S过程中Z环的正常形成和细胞形态。猪细胞分裂
Streptococcus suis(S. suis) is an important zoonotic pathogen that causes major economic losses in the pig industry worldwide. TheS. suiscell division process is an integral part of its growth and reproduction, which is controlled by a complex regulatory network. Pyruvate dehydrogenase (PDH), which catalyzes the oxidative decarboxylation of pyruvate to form acetyl-CoA, while reducing NAD + to NADH, plays an important role in energy metabolism. Recently, we reported thatpdhregulates virulence by reducing stress tolerance and biofilm formation inS. suisserotype 2. In this study, we found that deletion of thepdhgene inS. suisresulted in abnormal cell chains, plump morphology and abnormal localization of the Z rings, indicating that the knockout mutant is impaired in its ability to divide. In addition, the interaction between FtsZ and PDH in vitro was confirmed by ELISA, and qRT-PCR analysis revealed that the deletion of thepdhgene results in differential expression of the division-related genesftsZ, ftsK, ftsl, zapA, divIC, pbp1a, rodA, mreD,andsepF. These results indicate thatpdhis involved in the normal formation of Z rings and cell morphology duringS. suiscell division.
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DOI：
--
发表时间：
2016-02
期刊：
Wei sheng wu xue bao = Acta microbiologica Sinica
影响因子：
--
作者：
Yujia Huo;Qiaonan Lu;Xiaowei Zheng;Yuanfang Ma;Feng Lu
通讯作者：
Yujia Huo;Qiaonan Lu;Xiaowei Zheng;Yuanfang Ma;Feng Lu
影响因子：
3.6
作者：
Addinall, SG;Lutkenhaus, J
通讯作者：
Lutkenhaus, J
影响因子：
1.5
作者：
A. Zeeman;-H.;Luttik;C. Thiele;Johannes;-P.;Van Dijken;Yde Steensmal
通讯作者：
A. Zeeman;-H.;Luttik;C. Thiele;Johannes;-P.;Van Dijken;Yde Steensmal
影响因子：
3.5
作者：
Ortiz C;Kureisaite-Ciziene D;Schmitz F;McLaughlin SH;Vicente M;Löwe J
通讯作者：
Löwe J
影响因子：
6.7
作者：
Albarracín Orio AG;Piñas GE;Cortes PR;Cian MB;Echenique J
通讯作者：
Echenique J