Two pathways regulate cortical granule translocation to prevent polyspermy in mouse oocytes.
Two pathways regulate cortical granule translocation to prevent polyspermy in mouse oocytes.
复制标题
两条途径调节皮质颗粒易位以防止小鼠卵母细胞的多精受精。
DOI：
10.1038/ncomms13726
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发表时间：
2016-12-19
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
卵子只能由一个精子受精。为了防止多精受精，环绕在哺乳动物卵子周围的透明带在受精后变得不能渗透，从而阻止更多的精子进入。受精后小带的结构变化是由皮质颗粒的胞吐作用驱动的。在减数分裂过程中，它们从卵母细胞的中心转移到质膜。然而，关于皮质颗粒移位的机制却知之甚少。在这里，我们使用Rab27a作为标记，研究活的哺乳动物卵母细胞中皮质颗粒的运输和动力学。我们证明了两种不同的机制驱动它们的运输：肌球蛋白Va依赖于肌动蛋白细丝的运动，以及一种意想不到的囊泡搭便车机制，通过这种机制，皮质颗粒与由肌球蛋白Vb驱动的Rab11a囊泡结合。抑制皮质颗粒的易位严重损害了精子进入的障碍，这表明易位缺陷可能导致由多精细胞引起的流产。哺乳动物的卵子释放皮质颗粒，以避免与多个精子受精，因为多精受精导致不能存活的胚胎。在这里，作者描述了推动小鼠卵子中颗粒转移到皮质的机制，并表明这一过程对于防止多精受精是必不可少的。
An egg must be fertilized by a single sperm only. To prevent polyspermy, the zona pellucida, a structure that surrounds mammalian eggs, becomes impermeable upon fertilization, preventing the entry of further sperm. The structural changes in the zona upon fertilization are driven by the exocytosis of cortical granules. These translocate from the oocyte's centre to the plasma membrane during meiosis. However, very little is known about the mechanism of cortical granule translocation. Here we investigate cortical granule transport and dynamics in live mammalian oocytes by using Rab27a as a marker. We show that two separate mechanisms drive their transport: myosin Va-dependent movement along actin filaments, and an unexpected vesicle hitchhiking mechanism by which cortical granules bind to Rab11a vesicles powered by myosin Vb. Inhibiting cortical granule translocation severely impaired the block to sperm entry, suggesting that translocation defects could contribute to miscarriages that are caused by polyspermy. Mammalian eggs release cortical granules to avoid being fertilized by more than a single sperm as polyspermy results in nonviable embryos. Here, the authors describe the mechanism driving translocation of the granules to the cortex in the mouse egg and show this process is essential to prevent polyspermy.
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影响因子：
9
作者：
Izidoro-Toledo, T. C.;Borges, A. C.;Araujo, D. D.;Leitao Mazzi, D. P. S.;Nascimento Junior, F. O.;Sousa, J. F.;Alves, C. P.;Paiva, A. P. B.;Trindade, D. M.;Patussi, E. V.;Peixoto, P. M.;Kinnally, K. W.;Espreafico, E. M.
通讯作者：
Espreafico, E. M.
DOI：
10.1083/jcb.201505086
发表时间：
2015-12-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Guimaraes SC;Schuster M;Bielska E;Dagdas G;Kilaru S;Meadows BR;Schrader M;Steinberg G
通讯作者：
Steinberg G
影响因子：
5.9
作者：
GRISCELLI, C;DURANDY, A;PRUNIERAS, M
通讯作者：
PRUNIERAS, M
影响因子：
2.7
作者：
DUCIBELLA, T;ANDERSON, E;RANGARAJAN, S
通讯作者：
RANGARAJAN, S
影响因子：
9.2
作者：
Azoury, Jessica;Lee, Karen W.;Verlhac, Marie-Helene
通讯作者：
Verlhac, Marie-Helene