Patronin regulates the microtubule network by protecting microtubule minus ends.
Patronin regulates the microtubule network by protecting microtubule minus ends.
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DOI：
10.1016/j.cell.2010.09.022
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发表时间：
2010-10-15
期刊：
影响因子：
64.5
通讯作者：
Vale RD
中科院分区：
文献类型：
--
作者：
Goodwin SS;Vale RD
微管蛋白组装成具有不同正负末端的微管聚合物。活细胞中的大多数微管+末端都是动态的；生长和收缩之间的转换由促进组装和破坏稳定的蛋白质调节。相反，负端通常不是动态的，这表明它们被一些未知的蛋白质稳定。在这里，我们已经确定Patronin(也称为ssp4)是一种稳定果蝇S2细胞中微管末端的蛋白质。在没有Patronin的情况下，负端通过kinesin-13微管解聚酶的作用失去亚基，导致稀疏的间期微管阵列和短的、无序的有丝分裂纺锤体。在体外，纯化的Patronin与微管负端的选择性结合足以保护它们免受Kinesin-13诱导的解聚。我们认为Patronin封顶并稳定微管负端，这一活动在微管细胞骨架的组织中起着关键作用。
Tubulin assembles into microtubule polymers that have distinct plus and minus ends. Most microtubule plus ends in living cells are dynamic; the transitions between growth and shrinkage are regulated by assembly-promoting and destabilizing proteins. In contrast, minus ends are generally not dynamic, suggesting their stabilization by some unknown protein. Here, we have identified Patronin (also known as ssp4) as a protein that stabilizes microtubule minus ends inDrosophilaS2 cells. In the absence of Patronin, minus ends lose subunits through the actions of the Kinesin-13 microtubule depolymerase, leading to a sparse interphase microtubule array and short, disorganized mitotic spindles. In vitro, the selective binding of purified Patronin to microtubule minus ends is sufficient to protect them against Kinesin-13-induced depolymerization. We propose that Patronin caps and stabilizes microtubule minus ends, an activity that serves a critical role in the organization of the microtubule cytoskeleton.
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