Developmental Expression of Vasopressin in the Human Hypothalamus: Double-Labeling with In Situ Hybridization and Immunocytochemistry
Developmental Expression of Vasopressin in the Human Hypothalamus: Double-Labeling with In Situ Hybridization and Immunocytochemistry
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人类下丘脑中加压素的发育表达：原位杂交和免疫细胞化学双重标记
DOI：
10.1203/00006450-199302000-00013
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发表时间：
1993
影响因子：
3.6
通讯作者：
R. Greenwood
中科院分区：
文献类型：
--
作者：
K. Murayama;R. Meeker;S. Murayama;R. Greenwood
摘要：用精氨酸加压素(VP)及其基因表达的免疫细胞化学(ICC)和原位杂交(ISH)相结合的方法，研究了精氨酸加压素(VP)及其mRNA在人下丘脑发育过程中的表达。22例患者采用常规福尔马林固定尸检材料，年龄从妊娠18周到21岁不等。所有受检者的视上核、视上副核和侧脑室旁核均可检测到VP-NeurPhyin II免疫反应细胞。免疫细胞化学标记细胞的平均大小在出生前增加，并在出生后保持不变。妊娠21wk时，细胞内首次检测到Vp基因；ICC检测到第一个可检测到的Vp后3wk。妊娠27wk后，VP-NeuroPhyin II和VP mRNA双重标记的标本获得一致而强烈的信号。双标细胞分为三类：第1类，ISH和ICC均呈强阳性(大多数大细胞神经元)；第2类，ISH阳性，ICC弱或阴性(罕见且多见于较年轻胎儿)；第3类，ICC阳性，ISH弱或阴性(主要分布于副核团)。因此，双重标记技术可以常规地用于人类尸检材料的冰冻或石蜡切片，以同时评估消息和多肽。在人类胚胎中，大细胞神经内分泌细胞中的VP mRNA相对较晚地出现在成人型水平，这表明与该系统中功能性突触相互作用的发展有关。
ABSTRACT: The developmental expression of arginine vasopressin (VP) and VP mRNA in human hypothalamus was studied using combined immunocytochemistry (ICC) for VP-neurophysin II and in situ hybridization (ISH) for VP mRNA. Routine formalin-fixed autopsy material was used from 22 cases ranging in age from 18 wk of gestation to 21 yr. VP-neurophysin II immunoreactive cells were detected in the supraoptic, accessory supraoptic, and paraventricular nuclei of all brains examined. The average size of the immunocytochemically labeled cells increased until birth and remained constant thereafter. VP mRNA was first detected in cells at 21 wk gestation; 3 wk after the first detectable VP by ICC. After 27 wk of gestation, consistent and strong signals were obtained from the specimens that were double-labeled for both immunoreactive VP-neurophysin II and VP mRNA. Three populations of double-labeled cells were identified: type 1, intensely positive for both ISH and ICC (most magnocellular neurons); type 2, positive for ISH and weak or negative for ICC (rare and generally found in younger fetuses), and type 3, positive for ICC and weak or negative for ISH (mostly scattered in accessory nuclei). Thus, double-labeling techniques can be routinely used on frozen or paraffin sections of human autopsy material for the simultaneous assessment of both message and peptide. In the human fetus, the relatively late appearance of adult-like levels of VP mRNA in the magnocellular neuroendocrine cells suggests an association with the development of functional synaptic interactions in this system.
视上核和室旁核个体大细胞神经内分泌细胞中加压素 mRNA 表达对缺水的反应。
影响因子：
4.1
作者：
Meeker,RB;Greenwood,RS;Hayward,JN
通讯作者：
Hayward,JN