Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection.
Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection.
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DOI：
10.1371/journal.pone.0187735
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Große-Brinkhaus C
中科院分区：
文献类型：
--
作者：
Pröll MJ;Neuhoff C;Schellander K;Uddin MJ;Cinar MU;Sahadevan S;Qu X;Islam MA;Poirier M;Müller MA;Drosten C;Tesfaye D;Tholen E;Große-Brinkhaus C
猪繁殖与呼吸综合征（PRRS）是一种传染病，会导致全球养猪业遭受巨大的经济和生产损失。这种疾病的发病机制取决于多种因素，其控制仍然存在问题。免疫系统通常防御传染病，尤其是树突状细胞（DC），它在病毒感染后激活免疫反应中发挥着至关重要的作用。然而，对 PRRS 病毒 (PRRSV) 免疫反应和遗传对免疫反应的影响的了解仍然不完整。鉴于此，我们使用 RNA 测序 (RNA-Seq) 研究了猪肺 DC 中宿主对 PRRSV 免疫反应的调节。在感染前（0小时）和感染不同时期（感染后3、6、9、12和24小时（hpi））收集来自两种不同猪品种（皮特兰和杜洛克）的肺DC。 RNA-Seq 分析总共揭示了 20,396 个预测的猪基因，其中包括品种特异性差异表达的免疫基因。皮特兰和杜洛克感染的肺树突状细胞在感染后的第一个时间点表现出相反的基因表达过程。在这些时期（3、6、9、12 hpi），杜洛克肺 DC 的反应比皮特兰肺 DC 更强烈、更明显。此外，聚类分析揭示了参与免疫相关途径的时间依赖性共表达基因组。确定了关键簇和通路，这有助于解释 PRRSV 感染后肺 DC 的生物学和功能背景，并表明 IL-1β1 作为重要的候选基因。 RNA-Seq 还用于表征每个品种的 PRRSV 病毒复制。 PRRSV 能够在上述两个品种的肺 DC 中进行不同的感染和复制。这些结果可用于研究生猪育种中的免疫性状和增强猪的健康。
The porcine reproductive and respiratory syndrome (PRRS) is an infectious disease that leads to high financial and production losses in the global swine industry. The pathogenesis of this disease is dependent on a multitude of factors, and its control remains problematic. The immune system generally defends against infectious diseases, especially dendritic cells (DCs), which play a crucial role in the activation of the immune response after viral infections. However, the understanding of the immune response and the genetic impact on the immune response to PRRS virus (PRRSV) remains incomplete. In light of this, we investigated the regulation of the host immune response to PRRSV in porcine lung DCs using RNA-sequencing (RNA-Seq). Lung DCs from two different pig breeds (Pietrain and Duroc) were collected before (0 hours) and during various periods of infection (3, 6, 9, 12, and 24 hours post infection (hpi)). RNA-Seq analysis revealed a total of 20,396 predicted porcine genes, which included breed-specific differentially expressed immune genes. Pietrain and Duroc infected lung DCs showed opposite gene expression courses during the first time points post infection. Duroc lung DCs reacted more strongly and distinctly than Pietrain lung DCs during these periods (3, 6, 9, 12 hpi). Additionally, cluster analysis revealed time-dependent co-expressed groups of genes that were involved in immune-relevant pathways. Key clusters and pathways were identified, which help to explain the biological and functional background of lung DCs post PRRSV infection and suggest IL-1β1 as an important candidate gene. RNA-Seq was also used to characterize the viral replication of PRRSV for each breed. PRRSV was able to infect and to replicate differently in lung DCs between the two mentioned breeds. These results could be useful in investigations on immunity traits in pig breeding and enhancing the health of pigs.
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DOI：
10.1007/978-1-4939-0888-2_10
发表时间：
2014
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Bagga S;Bouchard MJ
通讯作者：
Bouchard MJ
影响因子：
3.7
作者：
Badaoui B;Rutigliano T;Anselmo A;Vanhee M;Nauwynck H;Giuffra E;Botti S
通讯作者：
Botti S
DOI：
10.1111/j.1439-0388.1998.tb00341.x
发表时间：
1998-06-01
期刊：
JOURNAL OF ANIMAL BREEDING AND GENETICS-ZEITSCHRIFT FUR TIERZUCHTUNG UND ZUCHTUNGSBIOLOGIE
影响因子：
--
作者：
Halbur, PG;Rothschild, MF;Bruna, JD
通讯作者：
Bruna, JD
影响因子：
3.7
作者：
Flori L;Gao Y;Laloë D;Lemonnier G;Leplat JJ;Teillaud A;Cossalter AM;Laffitte J;Pinton P;de Vaureix C;Bouffaud M;Mercat MJ;Lefèvre F;Oswald IP;Bidanel JP;Rogel-Gaillard C
通讯作者：
Rogel-Gaillard C
影响因子：
4.4
作者：
Grayson, Mitchell H.;Ramos, Madeleine S.;Holtzman, Michael J.
通讯作者：
Holtzman, Michael J.