Stem cell enrichment with selectin receptors: mimicking the pH environment of trauma.
Stem cell enrichment with selectin receptors: mimicking the pH environment of trauma.
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DOI：
10.3390/s130912516
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发表时间：
2013-09-17
期刊：
影响因子：
--
通讯作者：
King MR
中科院分区：
文献类型：
--
作者：
Cao TM;Mitchell MJ;Liesveld J;King MR
造血干细胞和祖细胞(HSPC)的分离对于移植治疗和HSPC研究是至关重要的，然而目前的分离技术昂贵、耗时且结果多变。选择素包被的微管在从人骨髓中快速分离HSPC方面显示出了希望，但进一步纯化HSPC仍然是一个挑战。因此，我们提出了一种仿生的HSPC分离装置，以模拟创伤时的酸性血管微环境，从而提高L-选择素与其拮抗受体PSGL-1与HSPC的结合频率。在酸性pH条件下，L-选择素包裹的微管增强了CD34+HSPC的粘附性，表现为细胞滚动速度的降低和滚动通量的增加。动态光散射被用作一种新的传感器来证实L-选择素在酸性条件下的构象变化，正如先前分子动力学预测的那样。这些结果表明，模拟创伤的酸性条件可以诱导L-选择素的构象延长，这可以用于临床上基于流动的HSPC分离。
The isolation of hematopoietic stem and progenitor cells (HSPCs) is critical for transplantation therapy and HSPC research, however current isolation techniques can be prohibitively expensive, time-consuming, and produce variable results. Selectin-coated microtubes have shown promise in rapidly isolating HSPCs from human bone marrow, but further purification of HSPCs remains a challenge. Herein, a biomimetic device for HSPC isolation is presented to mimic the acidic vascular microenvironment during trauma, which can enhance the binding frequency between L-selectin and its counter-receptor PSGL-1 and HSPCs. Under acidic pH conditions, L-selectin coated microtubes enhanced CD34+ HSPC adhesion, as evidenced by decreased cell rolling velocity and increased rolling flux. Dynamic light scattering was utilized as a novel sensor to confirm an L-selectin conformational change under acidic conditions, as previously predicted by molecular dynamics. These results suggest that mimicking the acidic conditions of trauma can induce a conformational extension of L-selectin, which can be utilized for flow-based, clinical isolation of HSPCs.
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影响因子：
3.4
作者：
Mitchell, Michael J.;King, Michael R.
通讯作者：
King, Michael R.
影响因子：
9.3
作者：
Narasipura, Srinivas D.;Wojciechowski, Joel C.;King, Michael R.
通讯作者：
King, Michael R.
影响因子：
5.1
作者：
Huang, Z.;King, M. R.
通讯作者：
King, M. R.
影响因子：
2.9
作者：
Charles, Nichola;Liesveld, Jane L.;King, Michael R.
通讯作者：
King, Michael R.
影响因子：
5.5
作者：
Cook, DN
通讯作者：
Cook, DN