The nuclear receptor genes HR3 and E75 are required for the circadian rhythm in a primitive insect.
The nuclear receptor genes HR3 and E75 are required for the circadian rhythm in a primitive insect.
复制标题
原始昆虫中的昼夜节律需要核受体基因HR3和E75。
DOI：
10.1371/journal.pone.0114899
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Tomioka K
中科院分区：
文献类型：
--
作者：
Kamae Y;Uryu O;Miki T;Tomioka K
昆虫的昼夜节律是由转录/翻译反馈环路组成的生物钟产生的，其中CYCLE和CLOCK是激活timeless（tim）和period（per）等多种时钟基因转录的关键元件。尽管时钟（Clk）的转录调控已被深入研究，但对于周期（cyc）的调控却知之甚少。在这里，我们鉴定了孤儿核受体基因HR3和E75，它们分别是哺乳动物时钟基因Rorα和Rev-erbα的直系同源物，作为参与原始昆虫Thermobia Domestica cyc基因节律表达的因子。我们的结果表明，HR3 和 E75 是有节奏地表达的，它们的正常、有节奏的表达是运动节律持续存在所必需的。他们的 RNAi 不仅显着改变了 cyc 的节律转录，还改变了 tim 的节律转录。令人惊讶的是，HR3 的 RNAi 揭示了 Clk 的节律表达，表明该祖先昆虫物种拥有 cyc 和 Clk 基因节律表达的机制。当 HR3 或 E75 被敲低时，tim、cyc 和 Clk 或 tim 和 cyc 分别发生相位振荡，表明这两个基因在时钟基因之间的相位关系调节中发挥重要作用。有趣的是，HR3 和 E75 也被发现参与蜕皮的调节，这表明它们将生物钟和发育过程联系起来。
Insect circadian rhythms are generated by a circadian clock consisting of transcriptional/translational feedback loops, in which CYCLE and CLOCK are the key elements in activating the transcription of various clock genes such as timeless (tim) and period (per). Although the transcriptional regulation of Clock (Clk) has been profoundly studied, little is known about the regulation of cycle (cyc). Here, we identify the orphan nuclear receptor genes HR3 and E75, which are orthologs of mammalian clock genes, Rorα and Rev-erbα, respectively, as factors involved in the rhythmic expression of the cyc gene in a primitive insect, the firebrat Thermobia domestica. Our results show that HR3 and E75 are rhythmically expressed, and their normal, rhythmic expression is required for the persistence of locomotor rhythms. Their RNAi considerably altered the rhythmic transcription of not only cyc but also tim. Surprisingly, the RNAi of HR3 revealed the rhythmic expression of Clk, suggesting that this ancestral insect species possesses the mechanisms for rhythmic expression of both cyc and Clk genes. When either HR3 or E75 was knocked down, tim, cyc, and Clk or tim and cyc, respectively, oscillated in phase, suggesting that the two genes play an important role in the regulation of the phase relationship among the clock genes. Interestingly, HR3 and E75 were also found to be involved in the regulation of ecdysis, suggesting that they interconnect the circadian clock and developmental processes.
登录
查看更多内容
影响因子：
11.4
作者：
Allada, R;Kadener, S;Rosbash, M
通讯作者：
Rosbash, M
影响因子：
3.5
作者：
Meireles, ACA;Rivas, GBD;Peixoto, AA
通讯作者：
Peixoto, AA
影响因子：
3.5
作者：
Tong, YH;Guo, HN;Bittman, EL
通讯作者：
Bittman, EL
影响因子：
3.5
作者：
Guillaumond, F;Dardente, H;Cermakian, N
通讯作者：
Cermakian, N
影响因子：
2
作者：
SOKOLOVE, PG;BUSHELL, WN
通讯作者：
BUSHELL, WN