A soluble angiotensin II-binding protein from rabbit liver.
A soluble angiotensin II-binding protein from rabbit liver.
复制标题
来自兔肝脏的可溶性血管紧张素 II 结合蛋白。
DOI：
10.1016/0006-291x(88)90616-x
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发表时间：
1988
影响因子：
3.1
通讯作者：
Soffer,RL
中科院分区：
文献类型：
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作者：
Rosenberg,E;RaviKiron,MA;Soffer,RL
在100，000 × g兔肝上清液组分中检测到血管紧张素II结合活性。该馏分中的结合活性的总量基本上大于通过用毛地黄皂苷处理可以从肝颗粒中溶解的结合活性的总量。粗品可溶性结合活性在几个方面与从颗粒中纯化的结合蛋白相似。首先，结合需要对氯汞苯磺酸的存在，结合的血管紧张素II被二硫苏糖醇释放。其次，与放射性碘标记的血管紧张素II交联的负责蛋白质在还原变性状态下的分子量约为75，000。最后，豚鼠抗血清提出的结合蛋白，已从颗粒纯化反应相同的可溶性和溶解的活动。
An angiotensin II-binding activity has been detected in the 100,000 × g supernatant fraction of rabbit liver. The total amount of binding activity in this fraction was substantially greater than that which could be solubilized from hepatic particles by treatment with digitonin. The crude soluble binding activity resembled the binding protein which had been purified from the particles in several respects. First, binding required the presence of p-chloromercuriphenylsulfonic acid and bound angiotensin II was released by dithiothreitol. Second, the molecular weight of the responsible protein cross-linked to radioiodinated angiotensin II was about 75,000 in the reduced, denatured state. Finally, guinea pig antiserum raised against the binding protein that had been purified from particles reacted identically with the soluble and solubilized activities.
血管紧张素受体的定位和特性。
影响因子：
4.9
作者：
F. Mendelsohn
通讯作者：
F. Mendelsohn
通过兔主动脉的螺旋切割条区分两种形式的血管紧张素。
影响因子：
--
作者：
O. M. Helmer
通讯作者：
O. M. Helmer