Shigella sonnei Does Not Use Amoebae as Protective Hosts.
Shigella sonnei Does Not Use Amoebae as Protective Hosts.
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DOI：
10.1128/aem.02679-17
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发表时间：
2018-05-01
影响因子：
4.4
通讯作者：
Clements A
中科院分区：
文献类型：
--
作者：
Watson J;Jenkins C;Clements A
福氏志贺菌和宋内志贺菌引起全世界大多数志贺菌病病例。然而，它们的分布不同，S。sonnei在中高收入国家占优势，S.在低收入国家中占主导地位。对S. sonnei是它可以生存在变形虫，这可以提供一个保护环境的细菌。在这项研究中，我们证明，而S。sonnei和S.弗氏杆菌可以在与自由生活的卡氏阿米巴共培养时存活，细菌生长主要是在细胞外。所有志贺菌分离株经A.与嗜肺军团菌不同，嗜肺军团菌可以在细胞内复制。我们的数据表明S. sonnei不能利用变形虫作为保护性宿主来提高环境存活率。因此，S.需要考虑Sonnei出现。重要性志贺氏菌的分布密切反映了一个国家的社会经济状况。随着许多人口众多的国家从低收入国家向中等收入国家的转变，S。sonnei感染已经成为一个主要的公共卫生问题。理解为什么S。改善生活条件是开发减少接触这种病原体的方法的关键。我们表明，自由生活的变形虫不太可能是环境宿主的S。宋内氏，因为所有测试的志贺菌菌株被阿米巴吞噬和降解。因此，需要其他的情景来解释S的出现和持续。宋内氏感染。
Shigella flexneri and Shigella sonnei bacteria cause the majority of all shigellosis cases worldwide. However, their distributions differ, with S. sonnei predominating in middle- and high-income countries and S. flexneri predominating in low-income countries. One proposed explanation for the continued range expansion of S. sonnei is that it can survive in amoebae, which could provide a protective environment for the bacteria. In this study, we demonstrate that while both S. sonnei and S. flexneri can survive coculture with the free-living amoebae Acanthamoebae castellanii, bacterial growth is predominantly extracellular. All isolates of Shigella were degraded following phagocytosis by A. castellanii, unlike those of Legionella pneumophila, which can replicate intracellularly. Our data suggest that S. sonnei is not able to use amoebae as a protective host to enhance environmental survival. Therefore, alternative explanations for S. sonnei emergence need to be considered. IMPORTANCE The distribution of Shigella species closely mirrors a country's socioeconomic conditions. With the transition of many populous nations from low- to middle-income countries, S. sonnei infections have emerged as a major public health issue. Understanding why S. sonnei infections are resistant to improvements in living conditions is key to developing methods to reduce exposure to this pathogen. We show that free-living amoebae are not likely to be environmental hosts of S. sonnei, as all Shigella strains tested were phagocytosed and degraded by amoebae. Therefore, alternative scenarios are required to explain the emergence and persistence of S. sonnei infections.
影响因子：
3.4
作者：
Saeed A;Johansson D;Sandström G;Abd H
通讯作者：
Abd H