Rabies virus binding to cellular membranes measured by enzyme immunoassay
Rabies virus binding to cellular membranes measured by enzyme immunoassay
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通过酶免疫测定法测量狂犬病病毒与细胞膜的结合
DOI：
10.1002/mus.880080411
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发表时间：
1985
期刊：
影响因子：
3.4
通讯作者：
Abigail L. Smith
中科院分区：
文献类型：
--
作者：
T. Lentz;J. Chester;Robin J. J. Benson;E. Hawrot;G. H. Tignor;Abigail L. Smith
The binding of rabies virus to cellular membranes was measured using an enzyme‐linked immunosorbent assay (ELISA). Virus binding to membranes adsorbed to the wells of microtiter plates was detected with rabies virus antibody and alkaline phosphatase‐linked second antibody. The greatest degree of binding was to myotube, neuroblastoma, and salivary gland membranes; intermediate levels occurred in striated muscle and nerve membranes; and low levels of binding were found in other membranes, including those of most parenchymal organs. Binding of rabies virus to myotube membranes was saturable, dependent on pH (with an optimum of pH 6.0), facilitated by the divalent cations Ca++, Mn++, and Mg++, and was temperature dependent. Binding was greatly reduced by inactivation of virus with β‐propiolactone or treatment of virus with trypsin. In embryonic chick myotubes, total acetylcholine receptor content and acetylcholinesterase activity undergo marked changes during development, first increasing and then decreasing at the time of hatching. Binding of rabies virus followed a similar pattern, indicating that the virus may interact with the acetylcholine receptor or other surface molecules undergoing similar developmental changes.
影响因子：
56.9
作者：
LENTZ, TL;BURRAGE, TG;TIGNOR, GH
通讯作者：
TIGNOR, GH
对溶解的 HeLa 细胞膜受体结合 B 组柯萨奇病毒和脊髓灰质炎病毒的固相测定。
影响因子：
3.7
作者：
Krah,DL;Crowell,RL
通讯作者：
Crowell,RL