Cloning, expression and cross‐linking analysis of the murine p55 tumor necrosis factor receptor
Cloning, expression and cross‐linking analysis of the murine p55 tumor necrosis factor receptor
复制标题
鼠p55肿瘤坏死因子受体的克隆、表达及交联分析
DOI：
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发表时间：
1991
影响因子：
5.4
通讯作者：
B. Foxwell
中科院分区：
文献类型：
--
作者：
K. Barrett;D. Taylor;A. Cope;A. Kissonerghis;P. Gray;M. Feldmann;B. Foxwell
肿瘤坏死因子（TNF）通过高亲和力细胞表面受体介导其多效性作用。在人类中，分子克隆已经确定了两种不同的，独立的TNF受体（TNFR）的55和75 kDa。然而，目前尚不清楚TNF的多种效应是否在受体类型之间分离。在小鼠中，先前的研究已经显示TNFR的功能异质性，因为WEHI 164成纤维细胞系对鼠和人TNF的细胞毒性作用敏感，而鼠T细胞系CT6响应鼠而不是人TNF而增殖。在这项研究中，克隆的cDNA编码小鼠同源的p55 TNFR的报告。该受体以相同的亲和力结合鼠和人TNF，并在WEHI 164和许多其他细胞系上表达，但在CT6细胞上仅检测到低水平的mRNA，并且没有蛋白质。然而，CT6细胞表达通过交联分析鉴定的约75 kDa的第二TNFR，其也在WEHI 164细胞上发现，并且仅结合鼠TNF。这些研究证实，在小鼠中也有两种TNFR，并表明至少在这些细胞系中，不同受体之间可能存在细胞毒性和增殖反应的分离。
Tumor necrosis factor (TNF) mediates its pleiotropic effects via high‐affinity cell surface receptors. In man, molecular cloning has identified two distinct, independent TNF receptors (TNFR) of 55 and 75 kDa. It is unclear, however, whether the multiple effects of TNF are segregated between the receptor types. In the mouse, previous studies had shown functional heterogeneity of TNFR, since the WEHI 164 fibroblast line is sensitive to the cytotoxic effects of both murine and human TNF, whereas the murine T cell line, CT6, proliferates in response to murine but not human TNF. In this study, the cloning of a cDNA encoding the murine homologue of the p55 TNFR is reported. This receptor binds murine and human TNF with equal affinity and is expressed on WEHI 164 and a number of other cell lines, but only low levels of mRNA and no protein is detectable on CT6 cells. CT6 cells, however, express a second TNFR of approximately 75 kDa, identified by cross‐linking analysis, which is also found on WEHI 164 cells, and binds only murine TNF. These studies establish that there are also two TNFR in the mouse, and suggests that there may be segregation of the cytotoxic and proliferative responses between different receptors, at least in these cell lines.
植物血凝素刺激的人淋巴细胞中细胞周期依赖性基因的表达。
DOI：
10.1073/pnas.82.16.5375
发表时间：
1985
影响因子：
11.1
作者：
Kaczmarek,L;Calabretta,B;Baserga,R
通讯作者：
Baserga,R
HeLa 细胞肿瘤坏死因子受体的多聚结构。
DOI：
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发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Smith,RA;Baglioni,C
通讯作者：
Baglioni,C