Peripheral serotonin regulates postoperative intra-abdominal adhesion formation in mice.
Peripheral serotonin regulates postoperative intra-abdominal adhesion formation in mice.
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外周血清素调节小鼠术后腹腔内粘连形成
DOI：
10.1038/s41598-017-10582-w
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发表时间：
2017-08-30
影响因子：
4.6
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Bi J;Zhang S;Du Z;Zhang J;Deng Y;Liu C;Zhang J
本研究的目的是探讨外周血清素在小鼠术后腹腔粘连形成中的作用及其可能机制。在野生型和Tph 1 −/−小鼠中进行盲肠摩擦操作以建立腹腔内粘连形成模型。5-羟色胺的缺乏显着降低了粘连评分，体重减轻，粘连厚度以及胶原纤维和羟脯氨酸的粘附组织中的水平。与野生型小鼠相比，Tph 1 −/−小鼠在粘附组织中表现出更温和的炎症反应和氧化应激。5-羟色胺缺乏还可降低腹腔液中派-1和纤维蛋白原的水平，升高t-PA和t-PA/派的水平。此外，Tph 1 −/−组小鼠粘连组织中CD 34、VEGF、TGF-β和5-HT 2B受体的表达显著降低。此外，Tph 1 −/− +5-HTP组小鼠的粘连比Tph 1 −/−组小鼠严重，对氯苯丙氨酸（PCPA）可显著减轻WT小鼠的粘连形成。结论：5-羟色胺通过促进粘连组织中的炎症反应、氧化应激、纤溶系统紊乱、血管生成和TGF-β1的表达来调节术后腹腔粘连的形成。
The aim of the present study is to investigate the role and potential mechanisms of peripheral serotonin in postoperative intra-abdominal adhesion formation in mice. The caecum-rubbing operations were conducted for intra-abdominal adhesion formation modelling in wild-type and Tph1−/− mice. The deficiency of serotonin significantly decreased the adhesion scores, weight loss, and adhesion thickness as well as levels of collagen fibres and hydroxyproline in the adhesive tissues. The Tph1−/− mice exhibited a milder inflammatory response and oxidative stress in the adhesive tissues than did the wild-type mice. Moreover, the deficiency of serotonin reduced the levels of PAI-1 and fibrinogen, and raised the t-PA and t-PA/PAI levels in the peritoneal fluids. Moreover, the expressions of CD34, VEGF, TGF-β and 5-HT2Breceptor in the adhesive tissues were significantly decreased in the Tph1−/− group mice. Furthermore, the Tph1−/− +5-HTP group showed more severe adhesions than did the Tph1−/− group mice, and the p-chlorophenylalanine (PCPA) could markedly alleviated the adhesion formation in the WT mice. In conclusion, the present study showed that peripheral serotonin regulated postoperative intra-abdominal adhesion formation by facilitating inflammation, oxidative stress, disorder of the fibrinolytic system, angiopoiesis and TGF-β1 expression via the 5-HT2Breceptor in the adhesive tissues.
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影响因子：
4.6
作者：
Di Filippo C;Falsetto A;De Pascale V;Tufariello E;De Lucia D;Rossi F;D'Amico M;Cennamo A
通讯作者：
Cennamo A
影响因子：
2.4
作者：
Ambler, Dana R.;Fletcher, Nicole M.;Saed, Ghassan M.
通讯作者：
Saed, Ghassan M.
影响因子：
3.8
作者：
Cahill, Ronan A.;Wang, Jiang Huai;Redmond, H. Paul
通讯作者：
Redmond, H. Paul
影响因子：
13.3
作者：
diZeregal, GS;Campeau, JD
通讯作者：
Campeau, JD
影响因子：
2.2
作者：
Fonfara, Sonja;Hetzel, Udo;Kipar, Anja
通讯作者：
Kipar, Anja