Vigorous beating of Chlamydomonas axonemes lacking central pair/radial spoke structures in the presence of salts and organic compounds.
Vigorous beating of Chlamydomonas axonemes lacking central pair/radial spoke structures in the presence of salts and organic compounds.
复制标题
在盐和有机化合物存在的情况下，缺乏中心对/径向辐条结构的衣藻轴丝的剧烈跳动。
DOI：
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发表时间：
2000
影响因子：
--
通讯作者：
R. Kamiya
中科院分区：
文献类型：
--
作者：
T. Yagi;R. Kamiya
鞭毛的衣原体突变体缺乏中央对微管或径向辐条不跳动，然而，从这些突变体中分离出的轴丝被发现在ATP和各种盐，糖，醇，和其他有机化合物的存在下显示出强烈的弯曲运动。例如，从pf 18（一种缺少中心对的突变体）分离的总轴丝中，约有15%在10 mM MgSO（4）和0.2 mM ATP存在下以约22 Hz的频率跳动，而在相同浓度的ATP和≤ 5 mM或≥ 25 mM MgSO（4）下没有跳动。pf 18轴丝在相同条件下的搏动频率和波形与野生型轴丝相似。类似地，10-50%的轴丝在0.5 M蔗糖、2.0 M甘油或1.7 M[10%（v/v）]乙醇存在下跳动。运动的外观并没有相关的轴丝ATP酶的变化，但是，这些物质在这些浓度通常增加纳米尺度振荡（超振荡）在pf 18轴丝的振幅，以及ATP诱导的蛋白酶处理的轴丝滑动解体的程度。缺乏中央对和内臂动力蛋白的各种亚种的双突变体的轴丝也在MgSO（4）浓度增加时跳动，但缺乏外臂动力蛋白和中央对的轴丝不跳动。这些和其他观察结果表明，小分子通过水活度或渗透压的某些变化扰乱微管滑动的调节。轴丝必须具有内在的能力，在各种非生理溶液条件下，在没有中心对/径向辐条的情况下跳动，只要外部动力蛋白臂存在。显然，中央对/径向辐条结构的主要功能是在生理条件下恢复这种活动。
Flagella of Chlamydomonas mutants lacking the central pair of microtubules or radial spokes do not beat; however, axonemes isolated from these mutants were found to display vigorous bending movements in the presence of ATP and various salts, sugars, alcohols, and other organic compounds. For example, about 15% of the total axonemes isolated from pf18, a mutant lacking the central pair, displayed beating in the presence of 10 mM MgSO(4) and 0.2 mM ATP at about 22 Hz, while none beat with the same concentration of ATP and < or = 5 mM or > or = 25 mM MgSO(4). The beat frequency and waveform of beating pf18 axonemes were similar to those of wild type axonemes beating under the same conditions. Similarly, 10-50% of the axonemes beat in the presence of 0.5 M sucrose, 2.0 M glycerol, or 1.7 M[10% (v/v)] ethanol. The appearance of motility did not correlate with the change in axonemal ATPase; however, these substances at those concentrations commonly increased the amplitude of nanometer-scale oscillation (hyper-oscillation) in pf18 axonemes, as well as the extent of ATP-induced sliding disintegration of protease-treated axonemes. Axonemes of double mutants lacking both the central pair and various subspecies of inner-arm dynein also beat at increased MgSO(4) concentrations, but axonemes lacking outer-arm dynein in addition to the central pair did not beat. These and other observations suggest that small molecules perturb the regulation of microtubule sliding through some change in water activity or osmotic stress. Axonemes must have an intrinsic ability to beat without the central pair/radial spokes under a variety of non-physiological solution conditions, as long as the outer dynein arms are present. Apparently, the major function of the central pair/radial spoke structures is to restore this activity under physiological conditions.
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DOI：
10.1016/s0021-9258(17)45384-1
发表时间：
1990-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Honkanen;J. Zwiller;R. Moore;S. L. Daily;B. Khatra;M. Dukelow;A. Boynton
通讯作者：
R. Honkanen;J. Zwiller;R. Moore;S. L. Daily;B. Khatra;M. Dukelow;A. Boynton
DOI：
10.1016/0006-291x(89)92189-x
发表时间：
1989-03-31
影响因子：
3.1
作者：
ISHIHARA, H;MARTIN, BL;HARTSHORNE, DJ
通讯作者：
HARTSHORNE, DJ
衣藻中轴丝 1 型磷酸酶对鞭毛动力蛋白的调节。
影响因子：
4
作者：
Habermacher,G;Sale,WS
通讯作者：
Sale,WS
低 ATP 条件下的重新激活可区分不同类别的麻痹鞭毛突变体。
影响因子：
--
作者：
Frey,E;Brokaw,CJ;Omoto,CK
通讯作者：
Omoto,CK