Cytotoxicity and ion release of alloy nanoparticles.
Cytotoxicity and ion release of alloy nanoparticles.
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DOI：
10.1007/s11051-011-0686-3
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发表时间：
2012-01
影响因子：
2.5
通讯作者：
Barcikowski, Stephan
中科院分区：
文献类型：
--
作者：
Hahn, Anne;Fuhlrott, Jutta;Loos, Anneke;Barcikowski, Stephan
关键词：
众所周知，纳米颗粒可能会对细胞产生毒性作用。由于磨损或生产故障，镍钛或钴铬制成的心血管植入物可能会释放出具有未知健康风险的合金纳米颗粒。我们显示了暴露于不同浓度的金属和合金纳米颗粒的人原代内皮细胞和平滑肌细胞的生物反应。使用激光烧蚀在三种不同的溶液中产生具有5-250 nm范围内的初级粒度的纳米颗粒，避免了人工化学添加剂，并且获得了含有仅由生物配体稳定的纳米颗粒的制剂。发现内皮细胞比平滑肌细胞对纳米颗粒暴露更敏感。钴和镍纳米颗粒引起的细胞毒性最高。相比之下，钛、镍-铁和镍-钛纳米颗粒在低于10 μM的纳米颗粒浓度下对细胞几乎没有影响。半胱氨酸中的纳米颗粒几乎完全溶解，而当纳米颗粒稳定在水或柠檬酸盐溶液中时，释放的离子较少。半胱氨酸稳定的纳米颗粒对细胞的抑制作用较小，表明半胱氨酸在介质中与生物活性离子形成金属络合物。
It is well-known that nanoparticles could cause toxic effects in cells. Alloy nanoparticles with yet unknown health risk may be released from cardiovascular implants made of Nickel–Titanium or Cobalt–Chromium due to abrasion or production failure. We show the bio-response of human primary endothelial and smooth muscle cells exposed to different concentrations of metal and alloy nanoparticles. Nanoparticles having primary particle sizes in the range of 5–250 nm were generated using laser ablation in three different solutions avoiding artificial chemical additives, and giving access to formulations containing nanoparticles only stabilized by biological ligands. Endothelial cells are found to be more sensitive to nanoparticle exposure than smooth muscle cells. Cobalt and Nickel nanoparticles caused the highest cytotoxicity. In contrast, Titanium, Nickel–Iron, and Nickel–Titanium nanoparticles had almost no influence on cells below a nanoparticle concentration of 10 μM. Nanoparticles in cysteine dissolved almost completely, whereas less ions are released when nanoparticles were stabilized in water or citrate solution. Nanoparticles stabilized by cysteine caused less inhibitory effects on cells suggesting cysteine to form metal complexes with bioactive ions in media.
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影响因子：
1.1
作者：
Hahn, Anne;Barcikowski, Stephan
通讯作者：
Barcikowski, Stephan
影响因子：
11.4
作者：
Limbach, Ludwig K.;Wick, Peter;Stark, Wendelin J.
通讯作者：
Stark, Wendelin J.
影响因子：
38.3
作者：
Bhabra, Gevdeep;Sood, Aman;Case, Charles Patrick
通讯作者：
Case, Charles Patrick
影响因子：
6.7
作者：
Menendez-Manjon, Ana;Barcikowski, Stephan
通讯作者：
Barcikowski, Stephan
影响因子：
4.1
作者：
Horie, Masanori;Nishio, Keiko;Yoshida, Yasukazu
通讯作者：
Yoshida, Yasukazu