Influence of perivascular peptides on endoneurial blood flow and microvascular resistance in the sciatic nerve of the rat.
Influence of perivascular peptides on endoneurial blood flow and microvascular resistance in the sciatic nerve of the rat.
复制标题
血管周围肽对大鼠坐骨神经神经内血流和微血管阻力的影响。
DOI：
--
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
L. Ho
中科院分区：
文献类型：
--
作者：
D. Zochodne;L. Ho
1.在支配神经血管的轴突终末中发现了多种血管活性肽，但其功能尚不清楚。在肠系膜小动脉中，P物质（SP）和降钙素基因相关肽（CGRP）被认为在紧张性血管舒张中发挥作用。2.我们探索了神经外膜辣椒素、SP、CGRP、spantide（SP拮抗剂）和hCGRP（8 - 37）（CGRP拮抗剂）对大鼠坐骨神经内膜隔室中血流量（EBF）和微血管阻力（EMR）的影响，通过氢清除率测量。3.在单独的动物组中，与单独应用载体的神经外膜相比，神经外膜辣椒素诱导EBF迅速、强烈和长期增加，并降低EMR。还通过研究个体动物的系列清除率曲线证实了充血反应。4.神经外膜辣椒素未改变多纤维坐骨-胫骨运动传导。5.当与辣椒素联合给药时，hCGRP（8 - 37）完全阻断了充血反应，并使EMR增加至高于合并对照范围。Spantide也阻断了辣椒素反应。6.当单独给药时，神经外膜hCGRP（8 - 37）和spantide均使相同动物的EBF降低至低于对照测量值，并使EMR增加至高于对照测量值。7.在10（-5）M时，神经外膜CGRP而不是SP降低EMR。与SP诱导的血管舒张相比，CGRP动脉内给药的血管舒张作用更大且更长（8 - 37），但Spantide并未降低动脉内对CGRP的反应。8.研究结果表明，神经外膜肽能末梢介导血管舒张反应（特别是通过CGRP），增加“下游”神经内膜室的血流量。生理性肽释放（由SP和CGRP受体拮抗剂阻断）可能在维持紧张性血管舒张中很重要。
1. A variety of vasoactive peptides has been identified in the axon terminals innervating vasa nervorum but their function is unknown. In mesenteric arterioles, substance P (SP) and calcitonin gene‐related peptide (CGRP) have been postulated to have a role in tonic vasodilatation. 2. We explored the effect of epineurial capsaicin, SP, CGRP, spantide (SP antagonist), and hCGRP (8‐37) (CGRP antagonist) on blood flow (EBF) and microvascular resistance (EMR) in the endoneurial compartment of the rat sciatic nerve, as measured by hydrogen clearance. 3. Epineurial capsaicin induced a prompt, intense and prolonged increase in EBF and lowering of EMR as compared to epineurial application of the carrier alone in a separate animal group. The hyperaemic response was also confirmed by studying serial clearance curves in individual animals. 4. Multifibre sciatic‐tibial motor conduction was not changed by epineurial capsaicin. 5. When co‐administered with capsaicin, hCGRP (8‐37) completely blocked the hyperaemic response and increased EMR above the pooled control range. Spantide also blocked the capsaicin response. 6. When administered alone, both epineurial hCGRP (8‐37) and spantide lowered EBF below and increased EMR above the control measurements in the same animals. 7. At 10(‐5) M epineurial CGRP, but not SP lowered EMR. Vasodilatation from intra‐arterial administration of CGRP was much greater and was more prolonged compared with that induced by SP. hCGRP (8‐37), but not spantide reduced the intra‐arterial response to CGRP. 8. The findings suggest that epineurial peptidergic terminals mediate a vasodilatory response (particularly through CGRP) that increases blood flow in the ‘downstream’ endoneurial compartment. Physiological peptide release (blocked by SP and CGRP receptor antagonism) may be important in maintaining tonic vasodilatation.
登录
查看更多内容
影响因子：
--
作者：
Phillip A. Low;T. Lagerlund;P. McManis
通讯作者：
Phillip A. Low;T. Lagerlund;P. McManis
影响因子：
21.1
作者：
S. H. Buck;T. Burks
通讯作者：
S. H. Buck;T. Burks
影响因子：
14.5
作者：
TUCK, RR;SCHMELZER, JD;LOW, PA
通讯作者：
LOW, PA
CGRP (8-37) 和 CGRP 受体脱敏抑制动脉周围神经刺激引起的肠系膜动脉床血管舒张。
DOI：
10.1016/0006-291x(90)92390-l
发表时间：
1990
影响因子：
3.1
作者：
Han,SP;Naes,L;Westfall,TC
通讯作者：
Westfall,TC