Cytofluorometric analysis for estrogen receptors using fluorescent estrogen probes.
Cytofluorometric analysis for estrogen receptors using fluorescent estrogen probes.
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使用荧光雌激素探针对雌激素受体进行细胞荧光分析。
DOI：
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
G. Barrows
中科院分区：
文献类型：
--
作者：
T. Kute;C. Linville;G. Barrows
乳腺癌组织的雌激素受体（ER）分析已被证明是非常有用的，在预测哪些患者将响应激素治疗，并有一个更好的预后。然而，ER测定是繁琐且耗时的。通过流式细胞术测量ER将是快速的，并且基于每个细胞的平均荧光-E2探针强度或每个细胞悬浮液中ER+细胞的百分比。对E2修饰的结构进行分析，通过竞争研究确定与ER的相对结合亲和力，并通过流式细胞术确定细胞悬浮液中的荧光摄取。缺乏对ER的高亲和力和化合物的纯度是荧光素标记的雌激素探针的主要问题。荧光素-E2化合物中酯键的碱水解通过HPLC证明，与存在的总组分相比，母体化合物中的雌二醇衍生物非常少。第二种类型的荧光雌激素，它有一个之间的类固醇和生色团的肽键也进行了测试。它污染较少，但无法进入细胞，也没有显示出与ER的结合活性。纯植物荧光雌激素香豆雌酚对ER的Ka为6 × 10（8）M-1，通过HPLC测定为单一组分。在ER+和ER-活细胞悬液上进行香豆雌酚的特异性荧光摄取。当这些香豆雌酚-细胞悬浮液在350-360 nm处激发并使用流式细胞术测量蓝色发射时，结果是荧光摄取，其不能被过量的非荧光E2探针高度取代。（250字处删节）
Estrogen receptor (ER) analysis of breast cancer tissue has been shown to be very useful in predicting which patients will respond to hormone therapy and have a better prognosis. The ER assay is, however, tedious and time consuming. Measurement of ER by flow cytometry would be rapid and based on either an average fluorescence-E2 probe intensity per cell or the percentage of the ER+ cells per cell suspension. Analysis of E2 modified structures for relative binding affinity to the ER determined by competition studies and for fluorescence uptake into cell suspensions determined by flow cytometry was performed. Lack of high affinity to the ER and purity of the compound were major problems for the fluorescein-labeled estrogen probes. Base hydrolysis of the ester linkage in fluorescein-E2 compounds demonstrated by HPLC very little estradiol derivative in the parent compounds compared to total components present. A second type of fluoresceinated estrogen which has a peptide bond between the steroid and the chromophore was also tested. It was less contaminated but was unable to get into the cell and showed no binding activity to the ER. A pure plant fluorescent estrogen, coumestrol, has Ka of 6 X 10(8) M-1 for the ER and is a single component as determined by HPLC. Specific fluorescent uptake of coumestrol was performed on ER+ and ER- viable cell suspensions. When these coumestrol-cell suspensions were excited at 350-360 nm and the blue emission was measured using flow cytometry, the result was a fluorescence uptake that was not highly displaceable by excess nonfluorescence E2 probes.(ABSTRACT TRUNCATED AT 250 WORDS)
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乳腺癌和前列腺癌中类固醇激素受体的组织化学分析。
DOI：
10.1177/28.8.7440959
发表时间：
1980
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Pertschuk,LP;Tobin,EH;Tanapat,P;Gaetjens,E;Carter,AC;Bloom,ND;Macchia,RJ;Eisenberg,KB
通讯作者：
Eisenberg,KB
用于 DNA 流式细胞术分析的组织的制备。
DOI：
10.1002/cyto.990010309
发表时间：
1980
期刊：
Cytometry
影响因子：
--
作者：
Thornthwaite,JT;Sugarbaker,EV;Temple,WJ
通讯作者：
Temple,WJ
雌激素受体的组织化学方法有效吗？
DOI：
10.1177/28.8.7003005
发表时间：
1980
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Chamness,GC;Mercer,WD;McGuire,WL
通讯作者：
McGuire,WL
人类和小鼠乳腺癌细胞与荧光雌酮结合。
影响因子：
11.2
作者：
Fisher,B;Gunduz,N;Zheng,S;Saffer,EA
通讯作者：
Saffer,EA