Calcitonin, angiotensin II and FPP significantly modulate mouse sperm function.
Calcitonin, angiotensin II and FPP significantly modulate mouse sperm function.
复制标题
降钙素、血管紧张素 II 和 FPP 显着调节小鼠精子功能。
作者：
L. Fraser;M. Pondel;G. Vinson
促受精肽（FPP）通过调节腺苷酸环化酶(AC)/cAMP通路，诱导获能依赖性反应，在无获能精子中刺激获能，然后在获能细胞中阻止获能，从而抑制自发顶体反应。与FPP一样，降钙素和血管紧张素II也存在于精浆中，因此可能影响精子功能；本研究考察了失能和失能小鼠精子对这三种肽的反应。降钙素（5ng /ml）和血管紧张素II（1和10nmol/l）与FPP （100nmol/l）一样，都能显著刺激获能，使用氯四环素（CTC）荧光和体外受精分析进行评估。两种或三种多肽的组合，在高或低、非刺激浓度下，比单独的多肽更具刺激作用，这表明它们可能作用于相同的信号通路，可能是AC/cAMP；初步数据表明降钙素确实刺激cAMP的产生。在失能细胞中，FPP和降钙素引起百日咳毒素对自发性顶体损失的敏感抑制，提示抑制G蛋白的参与；血管紧张素II无明显影响。当使用所有三种多肽时，血管紧张素II不会干扰对FPP/降钙素的抑制反应。这些结果表明，血管紧张素II、降钙素和FPP可能以某种方式调节AC/cAMP信号转导通路，但其确切机制尚未阐明。
Fertilization-promoting peptide (FPP) regulates the adenylyl cyclase (AC)/cAMP pathway to elicit capacitation-dependent responses, stimulating capacitation in uncapacitated spermatozoa and then arresting it in capacitated cells, thereby inhibiting spontaneous acrosome reactions. Like FPP, calcitonin and angiotensin II are found in seminal plasma and so might affect sperm function; this study investigated responses in uncapacitated and capacitated mouse spermatozoa to these three peptides. Both calcitonin (5 ng/ml) and angiotensin II (1 and 10nmol/l), like FPP (100nmol/l), significantly stimulated capacitation, assessed using chlortetracycline (CTC) fluorescence and fertilization in vitro analyses. Combinations of two or three peptides, at high and low, non-stimulatory concentrations, were more stimulatory than the individual peptides, suggesting that they may act on the same signalling pathway, plausibly AC/cAMP; preliminary data indicate that calcitonin does stimulate cAMP production. In capacitated cells, FPP and calcitonin elicited pertussis toxin-sensitive inhibition of spontaneous acrosome loss, suggesting involvement of inhibitory G proteins; angiotensin II had no detectable effect. When all three peptides were used, angiotensin II did not interfere with inhibitory responses to FPP/calcitonin. These results suggest that angiotensin II, calcitonin and FPP may somehow modulate the AC/cAMP signal transduction pathway, but the precise mechanisms involved have yet to be elucidated.
影响因子：
7.8
作者：
Huang, S;Deerinck, TJ;Ellisman, MH;Spector, DL
通讯作者：
Spector, DL
DOI：
10.1073/pnas.95.5.2552
发表时间：
1998-03-03
影响因子：
11.1
作者：
Hagaman, JR;Moyer, JS;O'Brien, DA
通讯作者：
O'Brien, DA