The dynamic organization of the perinucleolar compartment in the cell nucleus.
The dynamic organization of the perinucleolar compartment in the cell nucleus.
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细胞核中核酸隔室的动态组织。
DOI：
10.1083/jcb.137.5.965
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发表时间：
1997-06-02
影响因子：
7.8
通讯作者：
Spector, DL
中科院分区：
文献类型：
--
作者：
Huang, S;Deerinck, TJ;Ellisman, MH;Spector, DL
核仁周室（perinucleolar compartment，PNC）是细胞核中一种特殊的结构，主要位于核仁的周围。由RNA聚合酶III转录的几种小RNA（例如，YRNA、MRPRNA和RNasePH 1 RNA）和多聚嘧啶束结合蛋白（PTB; hnRNPI）迄今已在PNC中鉴定（Ghetti，A.，S. PinolRoma，W.M.迈克尔角Morandi和G.德莱弗斯1992. Nucleic Acids Res. 20：3671-3678; Matera，A.G.，M.R. Frey，K. Margelot和S.L.沃林1995. 129：1181-1193; Lee，B.，A.G. Matera，D.C. Ward，and J. Craft. 1996. Proc. Natl. Acad. Sci. USA. 93：11471-11476）。在本报告中，我们进一步表征了固定和活细胞中的这种结构。在大量人类癌症和正常细胞中检测PNC表明，PNC在癌细胞中更为普遍。通过使用单克隆抗体，SH 54，特异性识别PTB的免疫标记的细胞周期的分析表明，PNC解离在有丝分裂的开始和改革在后期末期的子核。为了使活细胞中的PNC可视化，产生PTB和绿色荧光蛋白（GFP）之间的融合蛋白。时间推移研究表明，PNC的大小和形状是动态的随着时间的推移。此外，在最佳固定的细胞中进行的电子显微镜检查显示，PNC由多条链组成，每条链的直径为180 -180 nm。有些链与核仁表面直接接触。此外，使用GFP-PTB融合蛋白的一系列缺失突变体对将PTB靶向PNC的序列要求的分析表明，融合蛋白靶向PNC需要在COOH或NH 2末端的至少三个RRM。这一发现表明，RNA结合可能是必要的PTB被定位在PNC。
The perinucleolar compartment (PNC) is a unique nuclear structure preferentially localized at the periphery of the nucleolus. Several small RNAs transcribed by RNA polymerase III (e.g., the Y RNAs, MRP RNA, and RNase P H1 RNA) and the polypyrimidine tract binding protein (PTB; hnRNP I) have thus far been identified in the PNC (Ghetti, A., S. PinolRoma, W.M. Michael, C. Morandi, and G. Dreyfuss. 1992. Nucleic Acids Res. 20:3671–3678; Matera, A.G., M.R. Frey, K. Margelot, and S.L. Wolin. 1995. J. Cell Biol. 129:1181–1193; Lee, B., A.G. Matera, D.C. Ward, and J. Craft. 1996. Proc. Natl. Acad. Sci. USA. 93: 11471–11476). In this report, we have further characterized this structure in both fixed and living cells. Detection of the PNC in a large number of human cancer and normal cells showed that PNCs are much more prevalent in cancer cells. Analysis through the cell cycle using immunolabeling with a monoclonal antibody, SH54, specifically recognizing PTB, demonstrated that the PNC dissociates at the beginning of mitosis and reforms at late telophase in the daughter nuclei. To visualize the PNC in living cells, a fusion protein between PTB and green fluorescent protein (GFP) was generated. Time lapse studies revealed that the size and shape of the PNC is dynamic over time. In addition, electron microscopic examination in optimally fixed cells revealed that the PNC is composed of multiple strands, each measuring ∼80–180 nm diam. Some of the strands are in direct contact with the surface of the nucleolus. Furthermore, analysis of the sequence requirement for targeting PTB to the PNC using a series of deletion mutants of the GFP–PTB fusion protein showed that at least three RRMs at either the COOH or NH2 terminus are required for the fusion protein to be targeted to the PNC. This finding suggests that RNA binding may be necessary for PTB to be localized in the PNC.
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DOI：
10.1083/jcb.112.5.785
发表时间：
1991-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ascoli CA;Maul GG
通讯作者：
Maul GG
影响因子：
14.9
作者：
GHETTI, A;PINOLROMA, S;DREYFUSS, G
通讯作者：
DREYFUSS, G
影响因子：
3.7
作者：
BRASCH, K;OCHS, RL
通讯作者：
OCHS, RL
DOI：
10.1016/s0022-5320(67)80074-1
发表时间：
1967-01-01
期刊：
JOURNAL OF ULTRASTRUCTURE RESEARCH
影响因子：
--
作者：
BOUTEILLE, M;KALIFAT, SR;DELARUE, J
通讯作者：
DELARUE, J
DOI：
10.1002/dvg.1020160107
发表时间：
1995-01-01
期刊：
DEVELOPMENTAL GENETICS
影响因子：
--
作者：
GALL, JG;TSVETKOV, A;MURPHY, C
通讯作者：
MURPHY, C