Iron and Zinc in the Embryo and Endosperm of Rice (Oryza sativa L.) Seeds in Contrasting 2'-Deoxymugineic Acid/Nicotianamine Scenarios.
Iron and Zinc in the Embryo and Endosperm of Rice (Oryza sativa L.) Seeds in Contrasting 2'-Deoxymugineic Acid/Nicotianamine Scenarios.
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DOI：
10.3389/fpls.2018.01190
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发表时间：
2018
影响因子：
5.6
通讯作者：
Álvarez-Fernández A
中科院分区：
文献类型：
--
作者：
Díaz-Benito P;Banakar R;Rodríguez-Menéndez S;Capell T;Pereiro R;Christou P;Abadía J;Fernández B;Álvarez-Fernández A
铁和锌缺乏症是世界范围内的营养失调症，可以通过生物强化方法增加水稻（Oryza sativa L.）谷物中的金属浓度来缓解。金属螯合剂烟胺（NA）的过量产生是最有效的螯合剂之一，但尚不清楚这是由于NA本身的富集和/或NA衍生物2 ' -脱氧氨基酸（DMA）的伴随富集。胚乳是稻谷中最常被消耗的部分，并介导营养物质从营养组织向富含金属的胚胎的转移。利用野生型水稻和6个过表达烟胺合成酶（OsNAS1）和/或大麦烟胺氨基转移酶（HvNAATb）的转基因品系，研究了不同水平的DMA和NA对水稻胚和胚乳中金属分布的影响。这些转基因株系概述了三种不同的DMA/NA情景：(i)在第一种情景中，由于利用NA合成DMA的能力有限（HvNAATb缺乏或低表达），NA水平的提高（通过OsNAS1的过表达）不会完全耗尽，并导致胚乳中NA、DMA、Fe和Zn以及胚胎中NA、DMA和Fe的一致富集；(ii)在第二个场景中,一个增强的NA水平(通过过度OsNAS1)将耗尽一个增强的能力使用NA DMA合成(通过表达HvNAATb),和结果在充实只有DMA和铁、胚乳和胚,和(3)在第三个场景中,缺乏足够的NA补给将限制DMA合成,尽管为此目的而使用NA增强的能力(通过表达HvNAATb),减少钠和结果,胚胎和胚乳中DMA变化不定，铁含量中度降低。此外，胚胎结构的定量LA-ICP-MS金属图谱图像显示，第一和第二种情况改变了Fe的局部分布，Zn的局部分布程度较小。讨论了DMA/NA水平在胚胎内铁和锌运输中的作用。
Iron and Zn deficiencies are worldwide nutritional disorders that can be alleviated by increasing the metal concentration of rice (Oryza sativa L.) grains via bio-fortification approaches. The overproduction of the metal chelator nicotianamine (NA) is among the most effective ones, but it is still unclear whether this is due to the enrichment in NA itself and/or the concomitant enrichment in the NA derivative 2′-deoxymugineic acid (DMA). The endosperm is the most commonly consumed portion of the rice grain and mediates the transfer of nutrients from vegetative tissues to the metal rich embryo. The impact of contrasting levels of DMA and NA on the metal distribution in the embryo and endosperm of rice seeds has been assessed using wild-type rice and six different transgenic lines overexpressing nicotianamine synthase (OsNAS1) and/or barley nicotianamine amino transferase (HvNAATb). These transgenic lines outlined three different DMA/NA scenarios: (i) in a first scenario, an enhanced NA level (via overexpression of OsNAS1) would not be fully depleted because of a limited capacity to use NA for DMA synthesis (lack of -or low- expression of HvNAATb), and results in consistent enrichments in NA, DMA, Fe and Zn in the endosperm and NA, DMA and Fe in the embryo; (ii) in a second scenario, an enhanced NA level (via overexpression of OsNAS1) would be depleted by an enhanced capacity to use NA for DMA synthesis (via expression of HvNAATb), and results in enrichments only for DMA and Fe, both in the endosperm and embryo, and (iii) in a third scenario, the lack of sufficient NA replenishment would limit DMA synthesis, in spite of the enhanced capacity to use NA for this purpose (via expression of HvNAATb), and results in decreases in NA, variable changes in DMA and moderate decreases in Fe in the embryo and endosperm. Also, quantitative LA-ICP-MS metal map images of the embryo structures show that the first and second scenarios altered local distributions of Fe, and to a lesser extent of Zn. The roles of DMA/NA levels in the transport of Fe and Zn within the embryo are thoroughly discussed.
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影响因子：
6.9
作者：
Banakar R;Alvarez Fernandez A;Díaz-Benito P;Abadia J;Capell T;Christou P
通讯作者：
Christou P
影响因子：
5.5
作者：
Ishimaru, Yasuhiro;Bashir, Khurram;Nishizawa, Naoko K.
通讯作者：
Nishizawa, Naoko K.
DOI：
10.1039/c39890000647
发表时间：
1989-05-15
影响因子：
--
作者：
ANDEREGG, G;RIPPERGER, H
通讯作者：
RIPPERGER, H
影响因子：
7.2
作者：
Ishimaru, Y;Suzuki, M;Nishizawa, NK
通讯作者：
Nishizawa, NK
DOI：
10.1038/nbt1091-957
发表时间：
1991-10-01
期刊：
BIO-TECHNOLOGY
影响因子：
--
作者：
CHRISTOU, P;FORD, TL;KOFRON, M
通讯作者：
KOFRON, M